MRTF-A和STAT3调控乳腺癌EMT及机制研究

项目名称： MRTF-A和STAT3调控乳腺癌EMT及机制研究

项目编号： No.31501149

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 廖兴华

作者单位： 武汉科技大学

项目金额： 20万元

中文摘要： 转移和复发是乳腺癌患者的主要死因，上皮细胞向间质细胞的转化(EMT)与乳腺癌转移关系密切。MRTF-A 和STAT3在包括乳腺组织在内的组织中广泛表达，通过调节细胞的迁移、侵袭，凋亡等生理过程影响肿瘤的发生、发展。在先前发现MRTF-A和STAT3协同促进乳腺癌的迁移的基础上，本研究将利用各种细胞和分子生物学方法，进一步确定MRTF-A和STAT3通过调控乳腺癌EMT过程促进乳腺癌迁移的确切分子机制。在分子和细胞及临床病理标本水平上，探究并阐明MRTF-A和STAT3通过调控乳腺癌EMT过程对乳腺癌发生的影响及机理。这些研究将揭示MRTF-A和STAT3在乳腺癌发生发展中的分子机制，为乳腺癌早期诊断和预后分析以及预防和治疗提供理论基础，并填补该领域国内研究空白。

中文关键词： 核受体；转录因子；信号转导；基因表达；信号间对话

英文摘要： Metastasis and recurrence are the major cause of breast cancer patient death. EMT is closely related with breast cancer metastasis. MRTF-A and STAT3 are widely expressed in the tissues of the body, including breast tissue. MRTF-A and STAT3 can regulate cell migration, invasion and apoptosis to control the physiological processes of tumor. In our previously study, we have found that MRTF-A and STAT3 synergistically promote the migration of breast cancer. Now, this study we will use variety methods of cell and molecular biology to further determine the molecular mechanism that MRTF-A and STAT3 promote breast cancer cell migration by regulating of breast cancer EMT process. And this study will demonstrate that MRTF-A and STAT3 regulate breast cancer EMT process to affect the occurrence of breast cancer and elucidate its molecular mechanism at the lever of the molecular and cellular and clinical pathological specimen. These studies will reveal the molecular mechanism of MRTF-A and STAT3 in breast cancer and provide a theoretical basis for early breast cancer diagnosis and prognosis and treatment so that it can fill the blank of domestic research in this field.

英文关键词： Nuclear receptor;Transcription factor;Signal transduction;Gene expression;Signal dialogue