分子氢防护辐射损伤的分子机理探索

项目名称： 分子氢防护辐射损伤的分子机理探索

项目编号： No.31270900

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 蔡建明

作者单位： 中国人民解放军第二军医大学

项目金额： 80万元

中文摘要： 自2007年国际上发现分子氢具有生物活性以来，氢气对放射损伤等多种疾病的防治作用相继被研究发现，呈现出极好的发展前景。但是，对其分子机理的研究却严重滞后，多个环节基本处于认识空白，难以解释它对多种疾病的显著防治作用，成为制约它由实验研究向临床应用转化的突出问题。本项目将选择分子氢发挥辐防护作用可能涉及的一些主要环节，研究它对各类自由基影响的特点和规律，它与自由基活性分子的电子结构信息及相互作用，它对氧化-还原酶活性的影响以及抗氧化作用，它对基因表达以及TLR4信号传导途径的影响，等等。本研究将首次从自由基活性、电子结构信息、氧化-还原酶、基因和蛋白改变、信号通路等多个层次，全方位揭示分子氢辐防护作用的分子机制，尤其要弄清它抗氧化的作用环节和抗氧化以外的其他重要作用机制。以期保持我国在分子氢辐射防护研究领域的领先地位，为促进分子氢向临床应用转化和拓展研究范围提供新理论和实验依据，具有重要科学

中文关键词： 分子氢；辐射防护；自由基；抗氧化酶；低剂量

英文摘要： Since 2007, Ohsawa et al discovered that molecular hydrogen (H2) acts as an antioxidant by selectively reducing hydroxyl radicals and peroxynitrite, there have been increasing reports which support H2 as a therapeutic gas against oxidative stress-related diseases, including ionizing radiation-induced injury. However the theory of antioxidant cannot explain all phenomenon in the biology of H2, as yet people have not found any other mechanism besides the reduction of hydroxyl radicals by H2. This study is designed to investigate the underlying radioprotective mechanism of H2 through several possible ways: Firstly, the potential regular of the reactions between H2 and free radicals, and the information of the electronic structure of the H2 and free radicals; Secondly, the effect of H2 in those enzymes which catalyze the oxidation-reduction reactions; Thirdly, the role of H2 in the gene-expression regulation and the TLR4 signaling transduction. This will be the first well-designed study on the biology of H2, including free radicals, electronic structure, oxidation-reduction reaction, gene expression and the signal transduction, put forward a comprehensive interpretation in the radioprotective mechanism of H2, especially in the researches tested molecular H2 not as an antioxidant. Our country will continue to be lead

英文关键词： hydrogen；radioprotection；free radical；antioxidant enzymes；low-dose radiation