功能化磁性纳米萃取新材料的优化合成研究及其在环境雌激素富集与检测中的应用

项目名称： 功能化磁性纳米萃取新材料的优化合成研究及其在环境雌激素富集与检测中的应用

项目编号： No.81502848

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 赵鸿雁

作者单位： 南京医科大学

项目金额： 18万元

中文摘要： 目前环境雌激素类物质分析测定中，存在目标物浓度低、基体干扰大等问题。为解决这一问题很多新型样品预富集材料不断被开发应用。由于聚吡咯和石墨烯都具有共轭大π键结构，使得他们对于大部分含有苯环的环境雌激素具有天生的亲和力。因此本项目选择这两种材料制备吸附性能强，易于分离的磁性萃取纳米材料，并在材料表面进一步功能化以富于它更好的分散性、亲水性及与介质的兼容性；以往材料合成过程均使用单因素优化，工作量巨大，若反应中各影响因素存在交互作用，更无法找到最优条件而导致合成效果很差，因此我们通过合成过程中系统的正交设计安排多因素实验，并引入matlab矩阵实验室中的遗传算法和神经网络进行及数值分析来优化合成条件，以制备性能良好的磁性材料。将该材料应用于环境及生物样本中对微量痕量的环境雌激素进行分离富集，并建立其相应的串联质谱检测方法，实现复杂基质中环境雌激素的高效快速的分析测定。

中文关键词： 环境雌激素；磁性纳米材料；磁性固相萃取；正交设计

英文摘要： Aiming at the problem in analysis and determine of environmental estrogens such as low concentration target compounds and strong interference and the development of new enriched materials, we proposed to prepare the magnetic nanoparticles for extraction with powerful adsorption capacity and the property of being prone to separation by using polypyrrole and grapheme which have natural appetency to environmental estrogens mostly containing benzene ring because of conjugated large πbond. The functionalization on the surface endowed the material with better dispersion, hydrophilicity and compatibility with medium. However, the synthesis process were optimized by single factor experiment previously which not only needed a heavy workload but also caused deficient synthetic effect when there existed interaction among factors, making it hard to find the optimum conditions. Hence, in order to prepare the magnetic materials with good performance, orthogonal experimental design was applied to conduct multi-factor experiments in the synthesis process and the synthesis conditions were optimized through genetic algorithm and neural network in matrix laboratory (MATLAB) and numerical analysis. The extraction conditions of the adsorption materials were optimized based on scientific experimental design and analysis and the synthesized magnetic particles enriched and separated the trace estrogens from environmental and biological samples. Besides, the corresponding detection method by tandem mass spectrometry was established, which realized the fast and efficient analysis determine of environmental estrogens in complex matrix.

英文关键词： environmental estrogen;Magnetic nano materials;Magnetic solid-phase;Orthogonal design