基于纳米纤维材料的循环肿瘤细胞高效捕获与释放研究

项目名称： 基于纳米纤维材料的循环肿瘤细胞高效捕获与释放研究

项目编号： No.51303140

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 一般工业技术

项目作者： 张南刚

作者单位： 武汉纺织大学

项目金额： 24万元

中文摘要： 恶性肿瘤是一类严重威胁人类生命健康的致命疾病，是中国成年人主要死因之一。肿瘤扩散复发是原发肿瘤不断转移的过程。循环肿瘤细胞（CTC）能扩散到人体不同的部位。 活检很难识别早期转移。CTC可作为肿瘤的液检标本，从而为潜在致命转移提供更早期和安全的检测渠道。CTC数量非常稀少(每毫升血几个到数百个)，因此捕获病人外周血中的CTC进而深入开展单肿瘤细胞的操控和分析是一项艰巨的挑战。 本项目将基于前期工作（用纳米纤维衬底成功实现胃癌和结直肠癌病人血样中CTC的捕获），接下来将开展基于MnO2纳米纤维衬的微流控型捕获芯片以克服现有捕获芯片无法对已捕获的CTC进行可控释放的缺点，并进一步提高捕获效率和简化操控流程。 本项目的成功实施将会拓展循环肿瘤细胞的研究，并将为癌症早期诊断、抗肿瘤药物药效评价、放化疗疗效分析、癌症的个体化治疗，新型抗癌药物筛选等研究提供有力手段和有价值的信息。

中文关键词： 微流控芯片制备；流速测量；循环肿瘤细胞；纳米纤维材料；细胞捕获与释放

英文摘要： Malignancies are a type of fatal diseases with serious threat to human health, which has become one of main causes of death for adults in China. There is a considerable body of evidence indicating that circulating tumor cells (CTCs) can travel to different tissues of the body, which is regarded as the cellular origin of metastases. In early stage metastasis, it is difficult to identify the metastatic sites for biopsy. CTCs can be regarded as the "liquid biopsy" of the tumor, thus providing a earlier and more safe access to potentially fatal metastases of tumor. However, detection and characterization of CTCs, as well as following manipulation and analysis of single CTC, have been technically challenging due to the extremely low amount ( few to several hundreds cells/mL) in the blood. This project is based on the previous work that CTCs have been successfully captured from blood samples of gastric and colorectal cancer patients by using electrospun TiO2 nanofiber structured substrates. Further research on CTCs will be expanded in MnO2 nanofibers-based CTC-capture microfluidic-device which can overcome the shortcoming of these current capture-devices that disable controllable release of captured CTCs, and improvement of CTC-capture efficiency and simplification of manipulation process. Successful implementation of

英文关键词： Microfluidic chip fabrication；Flow rate measurement；Circulating tumor cells；Nanofiber materials；Cell isolation & release