pH调控的循环肿瘤细胞捕获、释放及在线高通量分析微流控芯片

项目名称： pH调控的循环肿瘤细胞捕获、释放及在线高通量分析微流控芯片

项目编号： No.21505105

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 涂琴

作者单位： 西北农林科技大学

项目金额： 20万元

中文摘要： 作为一种重要的肿瘤转移标志物，循环肿瘤细胞（CTCs）的捕获与分析对恶性肿瘤的早期诊断、病情检测、治疗和预后具有重要意义。虽然细胞分离和富集方法的发展在一定程度上解决了CTCs分离和富集的难题。但是，由于这些方法固有的缺陷限制了其应用。为此，根据项目申请人多年在微流控芯片细胞功能分析及功能纳米材料制备等方面的研究工作基础上，立项研究pH调控的循环肿瘤细胞捕获、释放及在线高通量分析微流控芯片，即：设计、制备“双面”功能聚合物纳米微球和纳米纤维修饰的氧化铟锡电极，并以其为基底材料，构建微流控芯片阵列平台。通过芯片内多功能单元的集成及电化学检测技术的应用，在芯片内研究pH调控的CTCs的可逆捕获与释放，以及在线、高通量CTCs性质的新分析方法。藉此，对CTCs捕获、释放、以及高通量在线分析的相关难题进行探索与研究。

中文关键词： 循环肿瘤细胞；捕获与释放；在线分析；高通量；微流控芯片

英文摘要： As an important tumor marker, the capture and analysis of circulating tumor cells (CTCs) is of significance for the early diagnosis of malignant tumor, disease detection, treatment and prognosis. Although the development of cell separation and enrichment methods has resolved CTCs’ separation and enrichment to a certain extent, the inherent defects of these developed methods limit their actual applications. Therefore, based on the study and actual works on cells related microfluidic chips and functional nanomaterial preparation, herein, the project applicant plans to study a microfluidic chip for pH value-regulated the capture and release of CTCs and online assay in a high-throughput manner, namely: design and preparation of dual surface functional polymeric nanoparticle- and nanofiber-modified indium tin oxide electrode, and using the indium tin oxide electrode as the base material to construct the microfluidic array platform. Through the integration of multiple functional units within the chip and the application of electrochemical detection methods, the project applicant plan to study pH value-regulated CTC capture and release in the chip, as well as online and high-throughput assays of CTC properties. Based on the study, also, the project applicant plans to explore and investigate the new technologies and new analysis methods for the capture, release and high-throughput analysis of CTCs in an online manner.

英文关键词： circulating tumor cells;capture and release;online assay;high-throughput;microfluidic chip