新型软/硬仿生复合型软骨组织修复支架材料的制备与性能研究

项目名称： 新型软/硬仿生复合型软骨组织修复支架材料的制备与性能研究

项目编号： No.51273062

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 一般工业技术

项目作者： 周智华

作者单位： 湖南科技大学

项目金额： 80万元

中文摘要： 在现有的功能仿生支架材料研究中，普遍存在着类天然组织环境与机械强度不能兼顾的技术瓶颈。基于此，本项目拟构建一种新型"软/硬"仿生复合软骨组织修复支架材料，其中多孔型"硬"支架由乙二胺改性聚丙乙交酯（EMPLGA）组成，"软"支架由明胶和透明质酸复合水凝胶体系组成。采用共聚法乙二胺改性聚丙乙交酯，并研究成型参数对"硬"支架微结构和力学性能的影响规律；采用仿生法制备复合水凝胶，并对其组分、交联方法和交联条件进行优化；构建生物活性因子分步控释体系，研究其分步释放行为；利用毛细作用将水凝胶复合到"硬"支架中，采用体外模拟（水解和酶解）和体内降解方法，阐明仿生复合支架体内外的降解机制；探索仿生复合支架在动物体内外的作用模式，揭示软骨修复材料组成、结构和活性功能物质的引入对软骨再生修复的效果。通过本项目的研究，可为新型功能仿生支架材料的设计提供新思路和重要技术支持，并有助于揭示生命体的奥秘。

中文关键词： 仿生复合支架；多孔材料；水凝胶；生物活性设计；软骨组织修复

英文摘要： There are technical difficulties in keeping consistency between natural-like tissue environment and mechanical strength during fabricating functional bionic scaffold materials. A novel soft/hard bionic composite scaffold, with both excellent biological properties and matched mechanical strength with cartilage tissue cicatrization, is prepared for cartilage tissue repairing engineering, and the microstructure, physicochemical properties and biological properties are also investigated. The porous hard scaffold of EMPLGA is composed of poly(lactide-co-glycolide) (PLGA) modified by ethylenediamine, and the soft hydrogel system contains gelatin and hyaluronic acid. EMPLGA is obtained by polymerized using ethylenediamine as modifier; and the influence of molding conditions on the microstructure and mechanical properites of the hard scaffold materials is investigated. The hydrogels are developed using bionic method and the componment of the hydrogels, cross-linking method and cross-linking conditions are optimized. The controlled gradual release system loaded bioactive factors is obtained and the gradual release behavior is investigated. The bionic composite scaffolds are prepared by adding hydrogels into the EMPLGA porous hard scaffold using capillarity method, furthermore, the in-vitro and in-vivo degradation behavio

英文关键词： bionic composite scaffold；porous materials；hydrogel；bioactivity design；cartilage tissue repairing