环境胁迫调控副溶血弧菌整合结合元件自主传播的分子机理

项目名称： 环境胁迫调控副溶血弧菌整合结合元件自主传播的分子机理

项目编号： No.31271830

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 陈兰明

作者单位： 上海海洋大学

项目金额： 80万元

中文摘要： 副溶血弧菌(Vibrio parahaemolyticus)污染引发的水产品安全事件在我国微生物性食品安全事件中位居首位。然而,副溶血弧菌介导食源性疾病爆发流行的规律至今尚未阐明。本课题研究探索环境胁迫调控副溶血弧菌整合结合元件自主传播的分子机理,以副溶血弧菌MnSCn1及其携带的具有多药抗性的整合接合元件ICEVpCn1为研究对象,运用转录组学技术,分析环境胁迫在全局性水平对ICEVpCn1整合、切离、结合等基因转录的影响;研究不同环境条件下结合受体对ICEVpCn1自主转移活性的影响;利用基因敲除技术与蛋白质组学方法,获取环境因子调控ICEVpCn1的信号传导通路信息;初步构建环境胁迫调控ICEVpCn1自主传播的多维(宿主-整合结合元件-受体)基因调控网络。本课题的开展将为副溶血弧菌污染造成的食源性疾病的爆发流行及其预警的研究提供理论基础和技术支持,进而提升我国在该领域的水平。

中文关键词： 副溶血弧菌；整合接合元件；环境应激；基因调控；食品安全

英文摘要： Vibrio parahaemolyticus contamination in aquatic products was the major contributors to food safety issues caused by pathogenic bacteria in China. However, the mechanism underlying outbreak and prevalence of foodborne disease caused by V. parahaemolyticus has been largely unknown. In this project, the interdisciplinary methodology will be employed to investigate the impact of environmental stresses on the regulation of an integrative and conjugative element ICEVpCn1 with multiple drug resistance gene cassettes in V. parahaemolyticus MnSCn1, which was isolated from aquatic products in China. The draft genome map of V. parahaemolyticus MnSCn1 has been obtained. The genes involved in integration, conjugation and regulation of ICEVpCn1 will be investigated at a global level by transcriptome analyses under stress conditions with or without conjugation receptors. The differential expression regulation genes of ICEVpCn1 will be knocked out, and proteome of wild and mutant V. parahaemolyticus grown under stress conditions will be analyzed to uncover signal transmission pathways of environmental stress. Based on the results of this project, a three dimensional (host-ICEVpCn1-receptor) gene regulation web mediated by environmental stresses will be constructed, which will lead to new insights into molecular mechanisms unde

英文关键词： Vibrio parahaemolyticus；integrative and conjugative elements；environment stresses；gene regulation；food safety