针刺改善自发性高血压大鼠心肌肥厚及舒缩功能损害的机制研究

项目名称： 针刺改善自发性高血压大鼠心肌肥厚及舒缩功能损害的机制研究

项目编号： No.81473781

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 喻晓春

作者单位： 中国中医科学院针灸研究所

项目金额： 75万元

中文摘要： 长期高血压可导致以心肌肥厚为重要特征的心室重塑，心肌肥厚的病理主要表现为心肌细胞肥大、非心肌细胞增生和间质纤维化，同时可伴有左心室功能损害。迄今现代医学尚无办法完全阻滞高血压心肌肥厚（细胞肥大和心肌组织纤维化）及心衰的发展进程，寻找新的有效阻滞该进程的方法乃是医学界的研究热点。已知针灸可兴奋迷走神经、治疗高血压、改善心脏功能，为针灸抑制高血压心室肥厚及心脏功能损害提供了可能。 本项目拟以自发性高血压大鼠为研究对象，采用小动物B超、单个心肌细胞收缩与细胞内钙同步记录及分子生物学等技术，探讨与心肌肥厚和心功能受损密切相关的血管紧张素II系统、β1－肾上腺素受体、生长因子及细胞因子等在介导针刺抑制心肌肥大、心肌组织纤维化中的作用；并重点分析心肌细胞钙及其信号调节因子在介导针刺抑制高血压心功能损害、尤其是早期左室舒张功能损害中的作用。此方面的研究几乎未见报道，应具有较积极的临床启示意义。

中文关键词： 针刺；自发性高血压大鼠；心肌肥厚；细胞内钙；钙信号

英文摘要： Cardiac plasticity, characterized as myocardial hypertrophy, could be induced by long-lasting hypertention. Myocardial hypertrophy actually includes several phathological alterations such as myocyte hypertrophy, non-myocyte hyperplasia of and interstitial fibrosis, which may be accompanied with functional impairment of left ventricle. There are no any therapies of western medicine which could completely delay or stop the progress from hypertension to myocardial hypertrophy even to cardiac diastolic or systolic malfunction. Searching for new therapies to stop or delay the progress is currently the focus for medical study. Acupuncture was showed to excite parasympathetic nervous system and produce antihypertensive and cardioprotective effects, suggesting a possibility for acupuncture to inhibit the hypertensive myocardial hypertrophy and cardiac malfunction. Spontaneous hypertensive rats will be used in the proposed study as the animal model to determine if or not the Renin-angiotensin II-aldosterone system, β1-adrenoceptor, related growth factors and cytokines is/are involved in the mediation of the acupuncture-produced inhibition of myocyte's hypertrophy and myocardial fibrosis, by means of the techniques including Small Animal Ultrasound(Vevo 770) for detection of cardiac function in small animals, Myocyte Calcium and Contractility Recording System (Ionoptix) for simultaneous recording of intracellular calcium transient and contraction in single ventricular myocyte and Western Blotting etc.. The special attention will be pay to analyze the roles played by the intracellular calcium and its signaling components in mediating the attenuation of acupuncture on the hypertension-induced impairment of cardiac function, in particular the left ventricular diastolic malfunction which occurred usually earlier than the systolic malfunction. There is so far few study involved in the same topic as aforementioned, suggesting the clinical significance of the study proposed above.

英文关键词： acupuncture;Spontaneous hypertensive rats;myocardial hypertrophy;intracellular calcium;calcium signaling