壳聚糖季铵盐与线粒体相互作用的生物热动力学及其机理

项目名称： 壳聚糖季铵盐与线粒体相互作用的生物热动力学及其机理

项目编号： No.21503075

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 夏彩芬

作者单位： 湖北工程学院

项目金额： 21万元

中文摘要： 壳聚糖季铵盐因其水溶性好、带正电性强、细胞黏附性好，而成为具有应用价值的生物医用载体，也是近年来基因载体材料的研究热点之一。本项目拟在亚细胞水平上，以线粒体作为主要研究对象，采用微量热、呼吸耗氧等方法，建立壳聚糖季铵盐与线粒体相互作用的热动力学模型，获取相关的热动力学信息。采用光谱法、显微分析、流式细胞术、酶联免疫等多种先进技术手段相结合，研究壳聚糖季铵盐对线粒体形态、呼吸功能、膜渗透性转换、细胞色素c、活性氧等的影响，并进一步考察分子量、取代度对壳聚糖季铵盐与线粒体相互作用影响的规律性，揭示内在作用机理。该研究旨在深入了解壳聚糖季铵盐在生命体内的动态代谢过程中的影响途径和作用机制，为壳聚糖季铵盐作为医用载体的安全性，提供亚细胞水平的理论和实践依据，同时促进学科深层次的交叉渗透。

中文关键词： 壳聚糖季铵盐；线粒体；微量热；相互作用；机理

英文摘要： Chitosan quaternary ammonium salt was regarded as biomedical carrier with application because of its good water-solubility, high cationic potential, and strong cell adhesion. In addition, it was the research hotspots of genetic vecter in recent years. In this project, chitosan quaternary ammonium salt will be taken as the main system, and lots of research techniques, such as microcalorimetry, microscopy, spectroscopy, flow cytometry, enzyme link immunosorbent assayed (ELISA) etc. will be combined. With the help of thermokinetics, the biological effect and mechanism of chitosan quaternary ammonium salt on mitochondria will be investigated from structure and function on the level of subcellar organelle. The effect of molecular weight and degree of substitution of chitosan quaternary ammonium salt will be further explored. The thermokinetic model of the effect of chitosan quaternary ammonium salt on living metabolism will be established, and a lot of thermokinetic parameters will be obtained. The biological effect mechanism of chitosan quaternary ammonium salt , and the relationship between its stucture and thermokinetic properties, will be revealed. The study will provide a lot of important information on synthesis, application and biosafety eveluation of chitosan quaternary ammonium salt .

英文关键词： chitosan quaternary ammonium salt;mitochondria;microcalorimetry;interaction;mechanism