切伦科夫能量转移成像用于胃癌早期诊断的研究

项目名称： 切伦科夫能量转移成像用于胃癌早期诊断的研究

项目编号： No.81471717

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 丁杰

作者单位： 中国人民解放军第四军医大学

项目金额： 73万元

中文摘要： 分子影像理论与技术的高速发展为胃癌的早诊早治奠定了基础，光学成像中的切伦科夫成像以其价格低、成像快、敏感度高、探针容易制备等优点成为目前研究的热点，有望进入临床应用。新出现的切伦科夫能量转移成像是指将放射性核素的切伦科夫光能转移给另一荧光受体，然后用光学成像设备检测受体的发射光，它解决了切伦科夫信号穿透力弱的问题，弥补了切伦科夫荧光成像的不足。但目前胃癌尚缺乏特异性的靶向探针，适合临床应用的荧光染料也极少。前期工作中，我们筛选出了能与胃癌血管内皮细胞特异性结合的短肽GX1，具有临床应用前景；碳量子点是新近研究的热点荧光物质，由单一碳元素组成，低毒无免疫原性，适合生物体应用。本项目拟联合GX1和碳量子点制备放射性探针并进行体内外鉴定，用于切伦科夫能量转移成像，对胃癌进行早期诊断。旨在为切伦科夫能量转移成像的应用及GX1和碳量子点用于胃癌的早期诊断提供理论依据，为胃癌的诊断提供新策略与新方法。

中文关键词： 切伦科夫能量转移成像；胃癌；碳量子点；GX1

英文摘要： Gastric cancer is seriously endangering the health of Chinese people. It is the major disease that our country have to overcome independently. However, it is difficult to achieve early diagnosis with existing technologies. Cerenkov radiation energy transfer imaging(CRET) is providing a new technical method to solve this problem. Cerenkov radiation generated by decay of an isotope serves as the energy donor and a fluorophore constitutes the energy acceptor.It has more advantages than conventional optical imaging modalities. But there is yet no specific targeting probes for gastric cancer imaging, and most of the fluorescent dyes are poisonous to human. We had found a peptide GX1 (CGNSNPKSC) binding to vasculature endothelial cells of human gastric cancer. Therefore, photoluminescent Carbon dots are non-toxic to mice under any dose and have potential use in fluorescence imaging in vivo, tumor cell tracking, and others.So we will prepare the probe homing to the gastric cancer using the GX1 peptide, carbon dots and isotopes to perform the early diagnosis of gastric cancer.This study was designed to provide a theoretical basis for early diagnosis of gastric cancer with Cerenkov radiation energy transfer imaging and Carbon dots-GX1 peptide, and provide a new strategy and a new method for the early diagnosis of gastric cancer．

英文关键词： CRET;Gastric cancer;Carbon dots;GX1