基于组织脏器寄生虫病早期诊断的纳米金探针生物芯片的基础研究

项目名称： 基于组织脏器寄生虫病早期诊断的纳米金探针生物芯片的基础研究

项目编号： No.81460316

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 杨毅梅

作者单位： 大理大学

项目金额： 48万元

中文摘要： 近年,纳米金结构的使用被作为光学生物传感器结合检测的方法，在食品检测、生物医学等领域已展开广泛研究。纳米金棒独特的光学性质,在疾病诊断中的应用前景,成为国外临床研究热点，但尚未见有关用于寄生虫病检测研究报道。云南因多其民族不良饮食行为，成为我国寄生虫病高发区之一，早期确诊是治疗的关键，但组织脏器的寄生病，取材定点不易、难度较大、有创伤，确诊率极低，而血清学诊断在感染早期敏感性不高，导致这类寄生虫病的误诊漏检率极高，成为了临床的疑难杂症，直接影响了治愈率，而微量、快速正是纳米金检测技术的优势，本课题拟探索制备纳米金表面等离子共振基质生物芯片，应用于组织脏器寄生虫病早期诊断，研究其高效性及实用性。纳米金生物芯片的灵敏、特异、快速、高通量等特点显示了荧光标记、酶标记等常规技术无法比拟的优越性,研究结果将为纳米金生物芯片应用于感染性疾病的早期临床诊断、现场流行病学动态观察的进一步研究奠定科学基础。

中文关键词： 组织器官寄生虫病；早期诊断；纳米金；探针；生物芯片

英文摘要： In recent years, the structure of the nanogold used as optical biosensor detection method, in food detection, biomedical and other fields has been widely studied. Nanogold bar unique optical properties, application prospect in disease diagnosis, become clinical research hotspot abroad, but have not yet seen reports for parasitic disease detection research. Yunnan because of its national poor eating behaviors, become a parasitic disease in our country one of high incidence, early diagnosis is the key to the treatment, but the parasitism of tissue organs, based on fixed point not easy, difficult, trauma, diagnosis rate is extremely low, and serologic diagnostic sensitivity in the early infection is not high, lead to this kind of parasitic disease misdiagnosis miss rate is extremely high, become the clinical diseases of diagnosis is very difficult, directly affects the cure rate, and nanogold trace, nanogold labelling techique rapid detection technology advantage, this topic proposed to explore the preparation of nanogold substrate surface plasma resonance biochip, parasitic disease early diagnosis applied in tissue organs, study the efficiency and practicability. Nanogold biochip sensitive, specific, rapid and high flux characteristics shows that the fluorescent tags, enzyme mark the incomparable superiority, conventional technologies such as the results will be for application of nanogold biochip in early clinical diagnosis of infectious diseases, and dynamic observation of field epidemiology scientific basis for further research.

英文关键词： Parasitic Diseases on tissue organs;early diagnosis;nanogold;probe;biochip