抑制粘附，亦或诱导生长？- - 人工晶状体材料表面功能化修饰对LECs行为的调控研究

项目名称： 抑制粘附，亦或诱导生长？- - 人工晶状体材料表面功能化修饰对LECs行为的调控研究

项目编号： No.51203120

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 有机高分子材料学科

项目作者： 林全愧

作者单位： 温州医科大学

项目金额： 25万元

中文摘要： 人工晶状体植入后面临着后囊混浊高发生（即后发性白内障）的临床问题，主要是其表面晶状体上皮细胞的无规粘附生长所致。通过对材料进行表面设计可以对其表面细胞行为进行有效调控。本项目针对人工晶状体材料表面晶状体上皮细胞行为调控的关键问题，通过材料表面设计，获得可有效调控晶状体上皮细胞粘附行为的人工晶状体材料。即：将层层组装技术与可控交联技术相结合，利用光交联的高度可控性，构建较大模量范围内硬度可控的多层膜涂层，为研究人工晶状体材料表面硬度对晶状体上皮细胞粘附功能的影响规律提供有效的技术平台；通过对人工晶状体材料表面进行图案化处理，构建具有涂层硬度/细胞诱导分子图案化结构的人工晶状体材料表面，探索图案化表面对晶状体上皮细胞粘附、生长等功能的主动调控，为设计和构建人工晶状体材料表面性质调控晶状体细胞功能提供可靠的科学依据，并为人工晶状体材料表面设计抑制后发性白内障提供坚实的技术基础。

中文关键词： 人工晶状体；后发性白内障；表面改性；晶状体上皮细胞；仿生修饰

英文摘要： Posterior capsule opacification (PCO, also called secondary cataract) is a significant complication of intraocular lens (IOLs) implantation in cataract treatment. The key issue of PCO is the attachment and proliferation of the remaining lens epithelial cells (LECs) onto the IOLs surface after cataract surgery. Surface design of material provides an effective way to control the cell behavior on material surface. In this proposal, the surface property of IOLs was tailed to control the LECs attachment and proliferation via Layer-by-Layer (LbL) and microcontact printting (μCP) techniques. First, the photo-crosslinkable groups are to be incorporated into the polyelectrolyte to construct photo-crosslinkable multilayer films. Taking advantage of the high controllability of the photo-crosslinking reaction, the modulus of the multilayer films is to be regulated by the degree of photo-crosslinking. The relationship of IOLs surface rigidity and LECs behavior is to be investigated and so as to get a LECs resistant multilayer coating on IOLs surface. Then, patterned surface is to be constructed on the IOLs surface via the combination of LbL and μCP techniques. The rigidity or the cell growth factors is to be patterned by PDMS stamping on cell-resistant multilayer surface, aiming to induce the LECs regular growth. The relatio

英文关键词： Intraocular lens；Posterior capsular opacification；Surface modification；Lens epithelial Cell；Biomimic