黄东海沉积物细菌的分类及多样性研究

项目名称： 黄东海沉积物细菌的分类及多样性研究

项目编号： No.31200005

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 微生物学、植物学

项目作者： 张德超

作者单位： 中国科学院海洋研究所

项目金额： 25万元

中文摘要： 海底生物圈是最复杂的微生物栖息地，蕴藏着丰富的细菌资源，但我国黄东海沉积物中细菌物种资源和细菌群落的地理分布还不清楚。本课题以黄东海沉积物为研究对象。采用培养方法分离海洋细菌，并进行分类学和系统发育研究，根据多相分类原则分类鉴定潜在的细菌新物种，调查我国黄东海沉积物的细菌物种资源；采用基因文库和PCR-DGGE等非培养方法，研究不同站位细菌的群落结构，阐明黄东海沉积物中细菌群落的地理分布特征。本课题的开展将促进我国海洋细菌分类学的发展，为开发利用我国海洋细菌资源提供重要的科学依据。

中文关键词： 黄东海；分类；多样性；；

英文摘要： Benthic biosphere is the most complex habitat for microbe and contains abundant bacterial resource. However, bacterial species and geographical distribution of bacterial community in the sediment of the Yellow Sea and East China Sea are still little known. This study takes the benthic sediments collected from the Yellow Sea and East China Sea as research materials. Marine bacteria are isolated by culture-dependent methods and further characterized by the taxonomic and phylogenetic studies. The potential novel isolates are identified by a polyphasic taxonomic approach. The bacterial species resource in the sediment of the Yellow Sea and East China Sea will be investigated. Culture-independent methods(clone library and PCR-DGGE) are used to analyze community structure of marine bacteria among different sites. The geographical distribution of bacterial community in the sediment of the Yellow Sea and East China Sea will be illustrated. The implementation of this study will promote the development of marine bacterial taxonomy, and provide scientific basis for utilization of marine bacterial resource of our country.

英文关键词： Yellow Sea and East China Sea；diversity；taxonomy；；