维生素E调控肉鸡肌内脂肪沉积分子机制的研究

项目名称： 维生素E调控肉鸡肌内脂肪沉积分子机制的研究

项目编号： No.31501970

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 畜牧学与草地科学

项目作者： 孙艳发

作者单位： 龙岩学院

项目金额： 21万元

中文摘要： 肌内脂肪（IMF）含量影响鸡肉的嫩度、多汁性和风味，是鸡肉品质的决定因素之一。适宜的IMF含量可以改善鸡肉的风味品质。由于鸡的IMF含量遗传力较低，通过遗传选择很难有效地提高肉鸡的IMF含量，通过营养调控鸡肉IMF含量是一种切实可行的途径。维生素E作为动物机体的必需微量营养素，除了在繁殖、抗氧化、免疫等方面具有重要的生物学功能外，在调控动物脂肪代谢方面，特别是肌内脂肪代谢方面，也发挥着重要作用。本研究以AA肉鸡为模式动物，通过日粮中添加不同水平的维生素E，测定鸡IMF代谢规律和胸肌IMF含量极端个体的基因表达谱，构建维生素E调控鸡IMF沉积的分子调控网络，并利用（前）脂肪细胞和鸡肌卫星细胞共培养技术对关键节点基因和关键途径进行验证。从转录水平、细胞水平和组织水平系统地研究维生素E对鸡IMF代谢调控机制，为提高肉鸡IMF沉积和改善肉品质提供必要的理论基础。

中文关键词： 肉鸡；维生素E；肌内脂肪；分子机制；调控网络

英文摘要： The content of intramuscular fat (IMF) is one of the determinants of meat quality and influences the tenderness, juiciness and flavour of meat. Suitable IMF content can improve the meat quality of broilers. It is ineffective to increase the IMF content through the genetic selection because of the lower heritability of IMF content in chicken. In contrast, the nutrition regulation for IMF contents is a practical method. Vitamin E is an essential micronutrient for animals, which plays important physiological roles in reproduction, antioxidant and immune functions, and lipid metabolism, especially in regulation of IMF metabolism. In present study, the metabolism rules of the IMF were measured for the diets supplement of different levels vitamin E in broilers. The molecular regulatory networks for the vitamin E regulation of IMF deposit in boilers were constructed through the gene differential expression profile from the breast muscle between high and low IMF content individuals. The key genes and pathway of regulatory networks were validated by the co-culture technology of adipocyte and muscle satellite cell. Systematic study on the molecule mechanism of vitamin E regulation of intramuscular fat deposit in broilers was performed from the transcriptional, cellular and tissue levels to provide the theoretical basis on the improvement of the IMF deposit and meat quality.

英文关键词： broiler;vitamin E;IMF;molecular mechanism;gene regulatory network