黑果枸杞果实成熟介导花青素合成的分子机制解析

项目名称： 黑果枸杞果实成熟介导花青素合成的分子机制解析

项目编号： No.31470391

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 曾少华

作者单位： 中国科学院华南植物园

项目金额： 85万元

中文摘要： 茄科植物果实为人们提供了大量的、富含花青素等营养成分的可食性食物。尽管，花青素代谢途径的合成调控已经比较清楚；但是，果实成熟介导花青素合成的分子调控机制尚不明晰。茄科药用植物黑果枸杞(Lycium ruthenicum Murr.)果实成熟进程中不断加剧合成、积累特征性药效活性成分花青素。本项目拟以不同发育时期黑果枸杞果实为材料，采用转录组学、蛋白组学、代谢组学和生物信息学方法，研究黑果枸杞果实发育与成熟过程中的差异表达基因、差异表达蛋白和差异表达代谢物；阐明黑果枸杞果实代谢流流向花青素途径的代谢途径网络调控机制。采用系统生物学、实时定量PCR和RNAi转基因等方法，筛选、鉴定黑果枸杞果实成熟介导花青素合成的关键基因及其功能；为揭示果实成熟过程参与花青素合成的分子调控机制奠定基础。最终，为枸杞及园艺作物新品种遗传改良提供重要的基因资源奠定基础。

中文关键词： 黑果枸杞；花青素；组学；果实成熟；代谢调控

英文摘要： Solanaceae fruits are favorable and edible foods because of its richness in nutrients including anthocyanins. Although the biosynthetic and regulatory mechanism of anthocyanin pathway is clear, it remains unclear the molecular mechanism how fruit ripening modulates anthocyanin biosynthesis. As an important medicinal herbs, Lycium ruthenicum Murr. fruits increasingly intensify the biosynthesis and accumulation of characteristic, bioactive ingredients anthocyanins during fruit ripening. With the purpose of illuminating the network regulation mechanism introducing metabolic flux into anthocyanin pathway in L. ruthenicum fruits at the metabolic pathways level, this project intends to figure out the differentially expressed genes, proteins, and metabolites in ripening fruits using transcriptomics, proteomics, metabolomics and bioinformatics methods. In addition, candidate gene(s) linking fruit ripening and anthocyanin biosynthesis will be identified using systematic biology and quantitative real-time PCR methods. The novel function(s) of candidate gene(s) will be characterized using RNAi transgenic methods, which may further pinpoint the regulatory mechanism of fruit ripening controlling anthocyanin biosynthesis. Thus, this project will potentially provide an important gene resource for genetic improvement of horticultural crops including Chinese wolfberry.

英文关键词： Lycium ruthenicum Murr.;Anthocyanin;Omics;Fruit ripening;Metabolic regulation