疏水无机纳米晶的表面功能化修饰及其生物医学检测应用

项目名称： 疏水无机纳米晶的表面功能化修饰及其生物医学检测应用

项目编号： No.21275015

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 汪乐余

作者单位： 北京化工大学

项目金额： 85万元

中文摘要： 发光与磁性纳米晶在基因检测、免疫分析和癌症诊断（荧光成像和核磁共振成像）等领域倍受关注。发光与磁性优良、尺寸均匀、形貌规则、粒径小的无机纳米晶通常在疏水有机溶剂中制备，颗粒表面疏水，不适合生物医学检测应用。本研究利用溶剂热技术制备系列稀土掺杂发光纳米晶(如NaYF4，LaPO4，LaVO4等)、磁性纳米晶(如Fe3O4，Pt-Fe等)和量子点(如ZnS等)，借助超声乳化、表面聚合及分子置换方法，探索一些简便、通用的修饰技术，在不改变纳米晶的性能、形貌、尺寸和聚集状态的前提下，在疏水纳米晶表面包覆生物相容性大分子，改善纳米晶的水溶性和生物相容性。并在大分子表面进行生物标记物偶联，探索纳米晶在免疫分析、癌细胞与活体荧光成像及核磁共振成像中的应用。以期为癌症等重大疾病的早期诊断提供新材料、新技术、新方法。

中文关键词： 纳米探针；表面功能化修饰；成像；检测；

英文摘要： Due to their novel optical and magnetic properties, uniform shape and small particle size, the luminescent and magnetic nanocrystals have been drawn great interests in the biomedical applications such as DNA detection, immunoassay, cancer cell fluorescence imaging and magnetic resonance imaging. However, these inorganic nanocrystals are hydrophobic because they are usually synthesized in the oliec acid-oleylamine-1-octadecene mixture solvent, which limits greatly their biomedcial applictions. So, in the current project, the rare-earth-doped NaYF4, LaPO4, and LaVO4 luminescent nanocrystals, magnetic nanocrystals (Fe3O4, Pt-Fe) and quantum dots with novel properties as well as controllable shape and size will be prepared in the oil system. To make these inorganic nanocrystals water-stable, biocompatible, and bioconjugatable, some facile and verstatile surface modification strategies will be developed for the surface functionalization of these hydrophobic nanocrystals by coating with biopolymers. And then the biological labels such as antibodies, peptides and nucleic acids will be conjugated onto the coating shell to obtain the nanobiotags. Finally, these as-prepared nanobiotags will be utilized for the immunoassays, in vivo/in vitro fluorescence imanging and magnetic resonance imagning. In summary, we hope these r

英文关键词： nanoprobes；surface functionalization；imging；detection；