植物对多重非生物胁迫的代谢应答及交谈机制研究

项目名称： 植物对多重非生物胁迫的代谢应答及交谈机制研究

项目编号： No.31300331

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 孙彩霞

作者单位： 东北大学

项目金额： 22万元

中文摘要： 干旱、高盐、高温等非生物胁迫因子严重影响植物的生长繁育过程，是导致全球农业减产的重要因素。在逆境条件下，植物可通过代谢路径调整，重新组织代谢网络而达到新的代谢稳态并获得对逆境的耐受性。研究植物逆境代谢应答机制对于揭示其抗性稳态建成是至关重要的。但传统的研究方法难以澄清植物逆境代谢稳态网络及关键因子，近年来代谢组学的发展为本领域的研究提供了可靠的技术平台。本研究拟采用核磁共振、气相色谱飞行时间质谱、液相色谱串联四极杆飞行时间质谱以及电感耦合等离子体质谱等多种互补的代谢组学分析技术平台，并结合多维可视化的数据深度挖掘策略，对植物在干旱、高盐及高温单一胁迫及相关的双重、三重胁迫等不同方式的非生物胁迫下的代谢应答机制进行整合研究，尤其关注尚鲜有报道的植物响应多重非生物胁迫的可能的代谢交谈机制。这不仅为获取逆境生态学新知识提供了全新思路，而且为未来抗多逆境植物品种的选育提供可能的靶标具有重要意义。

中文关键词： 玉米；代谢组；干旱胁迫；盐胁迫；温度胁迫

英文摘要： Both growth and productivity of plants are adversely affected by various abiotic stress factors, such as drought, salt and high temperature. Abiotic stress in fact is the principal cause of crop reduction world wide. Such changes in growth condition require an adjustment of metabolic pathways and metabolic network in plant, aimed at achieving a new state of homeostasis, in a process that is usually referred to as acclimation. Researches focusing on responses of plants to various abiotic stresses are very important for understanding the resistance genesis. Recently, the high-throughput metabolomics provides a reliable technological platform for clarification of the key component for achieving the state of homeostasis and the metabolic network involving in those abiotic stresses. In this research, we will apply an unprecedented range of complementary metabolomic analytical platforms including quantitative proton NMR spectroscopy (1H-NMR), gas time-of-flight tandem mass spectrometry (GC-TOF-MS), liquid chromatography quadrupole time-of-flight tandem mass spectrometry (LC-QTOF-MS), inductively coupled plasma mass spectrometry (ICP-MS) and liquid chromatography coupled to photodiode array detection (HPLC-PDA) to study the metabolic response of plants to different abiotic stresses, such as single stress and multi-stre

英文关键词： Maize；Metabolome；Drought stress；Salt stress；Temperature stress