多功能核酸光催化可视化传感平台

项目名称： 多功能核酸光催化可视化传感平台

项目编号： No.21475013

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 张信凤

作者单位： 成都理工大学

项目金额： 82万元

中文摘要： 核酸在生物传感领域中应用广泛，其新功能的开发对传感技术的研究至关重要。本项目拟将染料小分子的光催化技术与核酸传感体系有机结合，提出核酸光催化新功能，在设计光催化装置、考察不同光源影响、优化染料小分子等的基础上，构建新型、高效的免标记核酸光催化体系，研究其光催化反应机理；并利用核酸光催化功能，设计信号开启、关闭型双链或单链核酸光催化可视化传感体系，旨在建成集分子识别、信号读出、信号放大等多个功能于一体的简单、高灵敏可视化传感平台，以期解决当前免标记、免分离核酸可视化传感体系难以直接用于超痕量目标物检测的问题；并利用该平台建立环境样品中超痕量污染物、生物样品中低丰度疾病相关蛋白质、病毒DNA等的可视化检测方法。本项目提出的核酸光催将是核酸的新功能之一，可为核酸传感技术的研究提供新的思路与方向；建立的多功能可视化传感平台将为人类健康、环境保护等领域提供简单、高灵敏的现场检测新技术。

中文关键词： 核酸；光催化；可视化检测；传感器

英文摘要： Nucleic acids are widely applied in sensing field, and thus the exploilation of their new fuctions is enssentially important for the development of biosensor. In this project, photo-catalytic technique will be integrated with nucleic acid for design of a novel nucleic acid-based photo-catalytic system and further exploilation of the new photo-catalytic function of nucleic acids. Various photo-catalytic apparatuses with different light sources will be constructed and the dye molecules will be optimized to fabricate a effective photo-catalytic system. Then, the mechanism of photo-catalytic reaction will be also studied. For detection of different analytes, double chain or single chain nucleic acid-based sensing systems with signal switch-on or switch-off will be designed. This sensing platform will be provided with multi-functions, i.e., target recognition, signal read-out and signal amplification. Thus, it will overcome the difficulty in detection of trace analytes in complicated samples when using the reported label-free and separation-free nucleic acid-based sensor. Finally, the sensing system will be applied for visual detection of pollutants, virus DNA, protein biomarkers, etc. The proposed nucleic acid-based photo-catalysis in this project will be a new function of nucleic acid, which would be helpful for designing of novel sensor and exploration of new research areas. Furthermore, the fabricated multi-functional sensing platform will provide a promising in-field detection technique for human health and environmental protection areas with simplicity and high sensitivity.

英文关键词： nucleic acid;photocatalysis;visual detection;sensor