骨骼肌损伤修复过程中巨噬细胞的作用及IGF-1/MGF干预效果与机制研究

项目名称： 骨骼肌损伤修复过程中巨噬细胞的作用及IGF-1/MGF干预效果与机制研究

项目编号： No.31271273

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 陈佩杰

作者单位： 上海体育学院

项目金额： 80万元

中文摘要： 骨骼肌损伤的修复一直是国际运动医学界研究的热点。近年来，有关对骨骼肌损伤修复机理的研究已不仅局限于肌卫星细胞本身，免疫细胞特别是巨噬细胞在肌卫星细胞活化及损伤修复中的重要性正逐步被认识，但其作用及作用机制目前仍处于初步探索阶段。骨骼肌严重损伤的临床治疗手段至今仍较为局限，尤其是缺乏科学有效的外源性药物进行局部干预以促进修复。因此，本研究通过构建骨骼肌钝挫伤动物模型，并借助在体巨噬细胞剔除技术，跟踪研究骨骼肌损伤修复过程中巨噬细胞以及相关的活性因子的变化、作用规律和调控机制；局部应用IGF-1和MGF这两种生长因子，对骨骼肌损伤后的修复进行干预，并观察其对损伤后修复进程的影响；离体状态下研究IGF-1和MGF对巨噬细胞功能的影响，以揭示巨噬细胞在骨骼肌损伤修复中的关键作用及其机制，并从巨噬细胞角度深刻揭示IGF-1和MGF参与骨骼肌损伤修复的可能机制，为进一步的临床研究及应用提供理论依据。

中文关键词： 骨骼肌；损伤；巨噬细胞；胰岛素样生长因子1；机械生长因子

英文摘要： 。 Repair of skeletal muscle injury has always been concerned in the field of sports medicine world widely. An important recent addition to this hot topic is that not only the satellite cells but the immune cells especially the macrophages may also play the important role during the repair process. However, the exact role and the regulation of macrophage in the repair are still not clear. Currently, the clinical treatment of severe skeletal injury is also limited and poorly developed by the absence of effective local pharmacologic intervention. In this project, we establish an animal model of skeletal injury, combined with an in vivo model of macrophage elimination, to investigate the role and the effects of macrophage and macrophage related factors during the repair process after skeletal muscle injury. Exogenous IGF-1 and MGF are also administrated locally to explore their effects and possible mechanisms in the intervention of the repair process. Moreover, the effects of IGF-1 and MGF on macrophage function are systemically studied by the in vitro model to further investigate the roles of IGF-1 and MGF in the repair of skeletal muscle injury from the view of macrophage. Our research project is expected to reveal the role and the possible regulation mechanism of macrophage in the repair of skeletal muscle i

英文关键词： Skeletal muscle；Injury；Macrophage；Insulin-like growth factor；Mechano growth factor