基于Caco-2细胞模型的蛋清肽结构与完整吸收关系研究

项目名称： 基于Caco-2细胞模型的蛋清肽结构与完整吸收关系研究

项目编号： No.31271907

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 刘静波

作者单位： 吉林大学

项目金额： 86万元

中文摘要： 肽的完整吸收（Intact absorption）是指某些肽可以不被消化道水解而以完整的形式被吸收。这种吸收对于功能肽在体内发挥作用有着重要的意义。鉴于目前肽的结构与完整吸收之间关系尚不明确，本项目组以蛋清肽为切入点，采用人结肠腺癌细胞（Caco-2）模拟肠道吸收，对氨基酸残基数大于3的肽的结构与完整吸收关系进行研究。在已经制备出的蛋清肽的基础上采用凝胶色谱，置换色谱等方法对完整吸收肽进行分离和结构鉴定，同时对已得到的116条功能肽进行完整吸收研究，通过以上两种方式得到完整吸收肽，通过Fomc固相合成技术进行结构改造，建立完整吸收肽谱库，采用3z、5z等描述符，建立肽的结构与表观吸收系数关系模型，采用分子模拟方法对吸收过程进行模拟，研究蛋清肽的结构与完整吸收之间的关系。本研究可建立完整吸收肽谱库，有望建立起较为公认的肽的结构与完整吸收之间关系，为食品源肽类的吸收机理研究奠定基础。

中文关键词： 蛋清；肽；吸收；构效关系；Caco-2细胞膜

英文摘要： The intact absorption of peptide means that peptides are able to be absorbed without previous hydrolysis. It has important significance for function peptides. The intact absorption of the peptide with more than 3 amino acid residues has not been widely accepted, while latest research show that some of those peptides can be absorbed intactly. According to that, our research group takes the egg white peptide as the starting point; build the absorption model with Caco-2 cells, focus on the relationship between the structure of peptide and the intact absorption. This project is going to build the database of the intact peptides through the separation of egg white peptides and structure modified of the found intact absorption peptides. The mathematical model of the relationship between the structure of peptide and the intact absorption will to be built with different descriptors. Molecular docking and molecular dynamics will be used to investigate the interaction between the peptide and the important protein. The project can build the database of the intact peptides and found the well accepted relationship between the structure of peptide and the intact absorption. It will contribute to the research on the mechanism of the intact absorption.

英文关键词： egg white；peptide；absorption；structure-activity relation；Caco-2 cell monolayer