杨树防御应答相关miRNA及其靶基因的鉴定与功能研究

项目名称： 杨树防御应答相关miRNA及其靶基因的鉴定与功能研究

项目编号： No.31200507

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 林学

项目作者： 陈英

作者单位： 南京林业大学

项目金额： 26万元

中文摘要： 杨树是我国重要的速生工业用材树种和绿化造林树种之一，对我国无性系林业可持续发展发挥了重要作用。随着速生高产杨树新品种的不断推广，各地杨树种植产业迅猛发展，但是林分结构单一，可能引发森林病害的爆发和流行，因此迫切需要研究杨树防御应答机制，为杨树抗性育种提供理论基础。miRNA(microRNA)是一类时序性调控生长发育进程的小分子RNA，在植物防御应答的精细调节中发挥重要作用。本研究基于杨生褐盘二孢菌全基因组序列信息和杨树基因组信息，以新一代半导体测序技术(PGM)为平台，开展杨树防御应答相关miRNAs及其靶基因的鉴定和功能研究，解析杨树-杨生褐盘二孢菌（Marssonina brunnea）相互作用的分子机制，挖掘和验证相关miRNAs及其靶基因的防御调控模式，分析杨树黑斑病防御反应中miRNAs的调控网络，为选育具有持久广谱抗性的杨树新品种提供理论依据。

中文关键词： 杨树；杨生褐盘二孢菌；miRNA；抗病；相互作用

英文摘要： Poplar is one of the important fast-growing timber and tree species which plays an important role in forestry sustainable development in China. The fast-growing planting of new varieties of high-yield poplar promotes the great development of poplar planting industries. But the poplar concentrated plantations and homogeneous forest structure is very likely to lead to forest disease outbreak and epidemic, so there is an urgent need to study the poplar defense mechanism and also can provide a theoretical basis for poplar disease-resistant breeding. miRNAs are now known to have greatly expanded roles in a variety of plant developmental processes and play an important role in the fine regulation of signal transduction, and the response to environmental stress and pathogen invasion. Based on Marssonina brunnea and poplar genome sequence resources, we take new-generation semiconductor sequencing technology as the starting point, to build and improve a platform to identificate and functional verificate poplar defense-related miRNAs and their target gene, to resolute the molecular mechanisms of interaction between poplar and sMarssonina brunnea, to excavate and validate of miRNAs and their target genes regulating defense mode, to analyze the regulation network of miRNAs in poplar disease-resistance, then to provide theo

英文关键词： poplar；Marssonina brunnea；miRNA；disease resistance；interaction