小麦组蛋白乙酰转移酶基因TaHAT1调控耐热性的分子机制解析

项目名称： 小麦组蛋白乙酰转移酶基因TaHAT1调控耐热性的分子机制解析

项目编号： No.31471478

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 胡兆荣

作者单位： 中国农业大学

项目金额： 89万元

中文摘要： 小麦是我国第三大粮食作物，高温是目前影响小麦产量和品质的主要限制因素之一，但小麦耐热性的分子机制迄今尚未阐述清楚。最近，我们实验室建立了小麦热胁迫前后的基因差异表达谱，鉴定出6560个差异表达基因，其中一个组蛋白乙酰转移酶相关基因TaHAT1在热胁迫后表达明显上调。在此基础上，本申请课题将以该基因为研究核心，从部分同源基因的克隆、表达模式分析、转基因功能鉴定以及调控的下游候选基因筛选等不同层面上开展研究，以期为阐明小麦耐热性的表观遗传调控机理奠定基础。具体研究目标为：获得普通小麦TaHAT1三个部分同源基因，分析部分同源基因在小麦高温胁迫前后的表达变化，获得小麦RNAi转基因株系，查明转基因植株与受体基因型在表型以及耐热性指标上的差异，筛选出2-3个重要的下游候选基因，初步解析TaHAT1在小麦高温胁迫过程中参与的调控途径。发表论文2-3篇，培养研究生2名。

中文关键词： 小麦；高温胁迫；组蛋白乙酰化；分子机制

英文摘要： Wheat (Triticum aestivum L.) is the third largest crop in China and the high temperature stress seriously affects its yield and quality, but the underlying mechanisms for heat stress tolerance in wheat is poorly understood. Recently, A total of 6560 probe sets were identified as heat responsive in wheat by using Affymetrix Genechip? Wheat Genome Array. Although epigenetic mechanisms are crucial to transcriptional regulation of gene expression, their involvement in response to heat stress is less clear. In this study, the main objectives are to figure out how histone acetyltransferase TaHAT1 functions in responsibility to heat stress in wheat. Our specific aims are: 1) Obtain the full-length cDNAs of three TaHAT1 homoeologs from wheat leaf and detect the homoeolog-specific expression pattern under heat stress. 2) Figure out the biological function of TaHAT1 with suppressed its expression in wheat. 3) Compare the heat tolerance between transgenic line and wild type. 4) Determine the downstream genes of TaHAT1 by ChIP-Seq. In summary, the expected data will provide some evidence that TaHAT1 might act as an important factor to heat stress in wheat.

英文关键词： Wheat;Heat Stress;Histone acetylation;Molecular basis