菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制

项目名称： 菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制

项目编号： No.31270534

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 葛菁萍

作者单位： 黑龙江大学

项目金额： 86万元

中文摘要： 微生态系统是研究物种共生及其与环境相互作用机制的理想模型。代谢物是细胞对环境变化的终端响应，代谢组学技术与其他系统生物学组学技术结合应用，有助于深入了解微生物菌群及所处生态系统的过程和功能。脱胶菌群的生长及关键酶代谢决定亚麻纤维生产周期及质量。本研究在脱胶菌株分离、产酶优化及沤麻系统菌群结构研究等前期工作基础上，以温水沤麻微生态系统为模型，采用代谢指纹分析、代谢轮廓分析等代谢组学技术，结合2D电泳等蛋白质组学技术和定量实时PCR及变性梯度凝胶电泳技术，研究菌群代谢物组、蛋白质组特征及环境基质动态变化，通过天然及加菌温水沤麻的对比试验，分析菌群结构、数量和物质转换的差异，探讨菌群演替、关键酶代谢及其与基质变化的一般规律，建立温水沤麻系统中脱胶菌群产酶代谢组学机制和不同关键酶产生菌群的共生机制。本研究是生态代谢组学概念及技术引入温水沤麻系统的有益尝试，对沤麻工艺改进具有实践指导意义。

中文关键词： 亚麻；高通量测序；代谢组学；细菌；群落结构

英文摘要： Microbial-ecosystem is an ideal model to understand the symbiosis of species and the reciprocity with their environment. Metabolites are the final response of cells to the changes of the environment. Metabolomics combining with other system biological 'omics' technologies are helpful for us to get further information about the process and function between microbial-community and their ecosystem. The growth of degumming bacteria and the metabolism of key enzymes jonitly determine the production cycle and quantity of flax fibres. The basic research has been finished, including separation of degumming bacteria, optimization of enzyme-producing condition and investigation of microbial-community structure. This present work takes the flax-retting microbial-ecosystem as a research model and aims at the feature of metabolome and proteome of the microbial-community, as well as the variation of environmental substances. Series of 'omics' and molecular technologies are planned to apply on this research, such as metabolomic fingerprinting, metabolomic profiling, two-dimensional electrophoresis, real-time quantitative PCR and denaturing gel gradient electrophoresis. According to the contrastive analysis on the variation of microbial-community structure and substance transition between natural flax-retting ecosystem and bact

英文关键词： Flax；High throughput sequencing；Metabonomics；Bacteria；Community structure