小鼠泡型包虫病浸润转移的动态代谢组学研究

项目名称： 小鼠泡型包虫病浸润转移的动态代谢组学研究

项目编号： No.81201315

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学四处

项目作者： 张颋

作者单位： 中国疾病预防控制中心寄生虫病预防控制所

项目金额： 23万元

中文摘要： 泡型包虫病是泡状棘球蚴寄生于人、兽体内引起的世界范围内危害严重的人兽共患寄生虫病。该病在我国西部青藏高原地区流行严重。早期诊断和治疗对病人的生存状况至关重要。由于对泡球蚴发生浸润转移的分子机制不清楚，目前尚缺乏有效的早期诊断方法，影响了防治效果。泡球蚴寄生于小鼠体内引起的病理过程与在人体内非常相似，分为早期浸润期，中期钙化期，晚期液化空洞期。本课题拟采用1H核磁共振技术，研究泡球蚴浸润转移过程早、中、晚三期小鼠的肝脏，血浆及尿液动态代谢组学，建立疾病进程代谢轮廓图谱，并采用主成分分析（PCA）联合偏最小二乘（PLAS-DA）多变量统计法对图谱进行判别，以筛选并鉴定疾病的内源性代谢标志物群；并运用生物信息学方法建立疾病相关的代谢途径及代谢网络，旨在分析泡球蚴浸润转移过程宿主内源性代谢物动态响应规律，并筛选早期标志物，为发展早期诊断方法，理解致病机制提供依据。

中文关键词： 泡型包虫病；代谢组学；早期诊断；核磁共振；标志物

英文摘要： Alveolar echinococcosis (AE), caused by the larval stage of Echinococcus multilocularis, is considered to be one of the most lethal parasitic zoonoses for humans worldwide. AE is highly endemic over the large areas of north-western provinces and autonomous regions of China. It was estimated that 91% new cases of AE per annum globally occurring in China. The national control program on echinococcosis has been launched in the country in 2005. In 2007, the central government listed echinococcosis as one of the six serious infectious diseases receiving free treatment. The infiltrative, tumor-like growth of the E. multilocularis metacestode usually affects the liver of intermediate hosts such as humans or rodents. In untreated or inadequately treated AE cases, the mortality could be high up to >90% within 10-15 years. Therefore, early diagnosis and treatment is a paramount measure in the control activity. However, in most cases, no clinical symptoms become evident until 10 or more years after the initial infection, when the disease is already well-advanced. The diagnosis of AE is currently based on the identification of cyst structures by imaging techniques, predominantly ultrasonography. However, the imaging techniques do not offer a good prospect for early diagnosis which reduced the quality of the management and t

英文关键词： alveolar echinococcosis；metabonomics；early diagnosis；nuclear magnetic resonance；marker