基于四波混频效应的悬挂芯光子晶体光纤DNA生物传感器研究

项目名称： 基于四波混频效应的悬挂芯光子晶体光纤DNA生物传感器研究

项目编号： No.61505115

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 无线电电子学、电信技术

项目作者： 谭晓玲

作者单位： 深圳信息职业技术学院

项目金额： 20万元

中文摘要： 利用四波混频效应中斯托克斯波或反斯托克斯波波长的漂移可以实现外界环境微小变化的探测，该方法灵敏度高、结构简单，在传感领域具有巨大研究价值和应用潜力。然而，普通光子晶体光纤（PCFs）空气孔小、非线性系数低等因素，限制了其在生物检测方面的进一步发展与应用。针对以上问题，本项目提出利用悬挂芯PCFs并结合四波混频效应来制作DNA生物传感器。理论上，研究悬挂芯PCFs中的四波混频效应，设计优化光纤结构与DNA生物层参数；实验上，解决DNA分子固定关键技术，制作出DNA传感探针，并搭建出基于悬挂芯PCFs的四波混频传感系统。最后，利用研制的传感器对生物样品进行初步检测，研究传感器的响应时间、检测限、检测范围等特性。本项目将悬挂芯PCFs的大空气孔、高非线性、灵活可控色散特性，与四波混频效应传感器的高灵敏特性相结合，传感器具有高灵敏度、快捷、灵活等优点，为生物医学中生物样品检测提供新的途径。

中文关键词： 微结构光纤传感；光子晶体光纤；四波混频；DNA

英文摘要： The four-wave mixing (FWM)-based sensor is utilizing the shift of FWM Stokes or anti-Stokes peaks to detect the minute changes of external environment. Due to its high sensitivity, simplicity, such a sensor exhibits great scientific research value and application potential in optical fiber sensing area. However, its application in bioassays is limited for small air holes and low nonlinear coefficient of photonic crystal fibers (PCFs). To overcome above problems, a DNA biosensor based on FWM effect of suspended-core PCF is proposed. In theory, the FWM effect in suspended-core PCF will be researched, and fiber structure and thickness of bimolecular layer will be designed and optimized. In experiment, the DNA sensor probe will be fabricated with immobilization methods of DNA molecules, and sensor system based on FWM effect of suspended-core PCF will be established. Finally, preliminary tests on biological samples will be taken with the fabricated sensor, and the response time, detection limit and detection range will be investigated with different concentrations of DNA samples. Combining high sensitivity of FWM-based sensors with the characters of large air holes, high nonlinear and flexibly controllable dispersion of suspended-core PCFs, a DNA sensing probe with advantages of high sensitivity, high speed and convenience is expected in this project, which provides a alternative method for bioassays in biomedicine.

英文关键词： microstructured fiber sensor;photonic crystal fiber;four-wave mixing;DNA