基于LC/MS、NMR分析方法的犊牛腹泻中兽医证候本质的代谢组学研究

项目名称： 基于LC/MS、NMR分析方法的犊牛腹泻中兽医证候本质的代谢组学研究

项目编号： No.31502113

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 水产学、兽医学

项目作者： 王胜义

作者单位： 中国农业科学院兰州畜牧与兽药研究所

项目金额： 20万元

中文摘要： 证侯是中兽医学理论体系中的重要概念，是辨证论治的起点和基石。但由于证侯的系统性、整体性和动态性特征，证侯诊断标准的客观界定以及症候的实质研究，一直是困扰中兽医药研究应用与发展的难题。本项目拟利用代谢物组学的理论及方法和系统生物学观点，为中兽医证侯本质的认识进行方法探索。项目首先明确湿热型犊牛腹泻和虚寒型犊牛腹泻的诊断标准，以临床自然腹泻犊牛病例为受试对象，采集血液和尿液，借助高效液相色谱-质谱联用、核磁共振现代分析技术手段，建立湿热型腹泻、虚寒型腹泻犊牛血清、尿液中内源性小分子代谢物谱，通过代谢组学模式识别分析，确定出特异性地关联湿热型腹泻和虚寒型腹泻犊牛的生物标记物，阐明犊牛腹泻中兽医证侯的微观本质。本研究结果将为中兽医证侯的诊断客观化和中兽医证侯本质的认识进行方法探索。

中文关键词： 犊牛腹泻；代谢组学；证候本质

英文摘要： Syndrome, which is an important concept in Traditional Chinese Veterinarian theory, is the starting point and cornerstone of diagnosis and treatment. But as a result of systematic, holistic and dynamic characteristics of syndrome, syndrome diagnostic criteria and the essence has always been a problem in application and development of Traditional Chinese Veterinarian Medicine(TCVM) .This project intends to explore a method for studying the syndrome essence of Traditional Chinese Veterinarian based on the Metabolomics theory and methods and systems biology methods. First refer to classic works and related literature, The diagnostic criteria of damp-heat type and cold type calf diarrhea will be established based on cluster analysis, factor analysis and other multivariate statistical methods. Then endogenous small molecule metabolites spectrum in serum and urine of calf with damp-heat type and cold type diarrhea will be established by the methods of LC-MS and NMR. The biological marker characterization associated with damp-heat type and cold type diarrhea syndrome will be determined by pattern recognition analysis ways of the Metabonomics, and the syndrome essential of damp-heat type and cold type diarrhea will be revealed. The results of this research will explore a method for building diagnostic criteria and revealing the essence of Chinese veterinarian syndrome.

英文关键词： calf diarrhea ;metabolomics;syndrome essence