根际促生菌Bacillus amyloliquefaciens SQR9与植物根系分泌物互作的分子机理研究

项目名称： 根际促生菌Bacillus amyloliquefaciens SQR9与植物根系分泌物互作的分子机理研究

项目编号： No.41271271

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 天文学、地球科学

项目作者： 张瑞福

作者单位： 南京农业大学

项目金额： 75万元

中文摘要： 根际有益微生物在促进植物生长及拮抗土传病害方面的应用日益受到重视，有效的根际定殖是其功能发挥的关键因素，根系分泌物作为植物与其他生物交流的"语言"，是根际环境中菌-植互作的信号分子的来源。本申请以实验室分离保存并获得实际应用的根际有益菌株Bacillus amyloliquefacien SQR9为材料，研究其与黄瓜根系分泌物互作的分子机理。SQR9表现了优异的根际定殖、生物膜形成、趋化性、多种拮抗物质分泌等性状，黄瓜根系分泌物能增强该菌的生物膜形成能力和趋化性。本研究拟阐释SQR9定殖对黄瓜根系分泌物组份的影响及其生物学意义，筛选黄瓜根系分泌物中调控SQR9根际定殖的信号分子，克隆鉴定SQR9中受信号分子调控的基因及其在根际定殖中的作用，以及它们响应信号分子的调控机制。为进一步增强B. amyloliquefacien SQR9在根际的定殖能力、更好的发挥其农业应用效果提供理论依据。

中文关键词： 根系分泌物；相互作用；根际定殖；生物膜形成；促生

英文摘要： Utilization of rhizospheric beneficial microbes for plant growth promotion and soil-borne diseases control has been paid more attentions. Efficient colonization of beneficial microbes on plant root plays vital role for their function performance, as the "language" of the plant to interact with other organisms, root exudates are the source of signals which regulate the interactions between bacteria and plants in rhizoshpere. This proposal will use beneficial strain Bacillus amyloliquefacien SQR9 as the model to investigate the molecular mechanism of its interaction with cucumber root exudates. B. amyloliquefacien SQR9 was isolated by our lab from the healthy cucumber rhizoshpere and applied in agricultural practice as the biofertilizer and biocontrol agent. B. amyloliquefacien SQR9 was a strong colonizer of cucumber root, and performed well for biofilm formation, chemotaxis activity and multiple antagnistic metabolites secretion, B. amyloliquefacien SQR9 showed positive chemotaxis response toward cucumber root exudates, which also increased the biofilm formation of B. amyloliquefacien SQR9. This study will investigate the effect of B. amyloliquefacien SQR9 colonization on the cucumber root exudates and its biological meaning, identify the signals of the root exudates which regulate the root colonization of B. amy

英文关键词： Root exudates；Interaction；Rhizosphere colonization；Biofilm formation；Growth promotion