基于新型共轭聚电解质-贵金属纳米复合体系的荧光生化传感研究

项目名称： 基于新型共轭聚电解质-贵金属纳米复合体系的荧光生化传感研究

项目编号： No.21475042

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 黄红梅

作者单位： 湖南师范大学

项目金额： 82万元

中文摘要： 本项目拟设计合成系列含有不同主链及側链（包括羧酸根、磺酸根、季铵盐等）的新型荧光共轭聚电解质。探索这些新型共轭聚电解质与贵金属（金、银、钯等）纳米材料经过电荷作用或原位合成形成复合体系，通过FRET、内滤效应等多种作用模式产生荧光信号变化的机制，发展基于新型纳米复合材料的传感技术，实现对环境和生命体系中多种离子、生物分子和细胞的检测。这类新型复合体系不仅拥有共轭聚合物优异的光电性质（如强的荧光和信号放大）和电解质的水溶性，而且拥有贵金属纳米材料的特征性,能有效地克服单一体系的荧光易受激发光强度、探针浓度、微环境和检测效率等众多可变或难以定量因素的干扰，从而实现在复杂体系（如生物体）中高灵敏、高选择性地准确获取化学与生物定量信息，为共轭聚电解质与贵金属材料的复合体系在医疗诊断、基因突变观测、环境检测以及国家安全防御等方面的应用提供新的物质基础和传感模式。

中文关键词： 荧光；化学/生物传感器；共轭聚电解质；贵金属纳米粒子

英文摘要： In this project, a series of novel conjugated polyelectrolytes containing different π-conjugated backbones and charged side chains (including carboxylate, sulfonate, quaternary ammonium salt) in their architectures will be designed and synthesized. Thus novel nanocomposite materials can be constructed with these new conjugated polyelectrolytes and noble metal nanoparticles (silver, gold and palladium,etc) by electrostatic interactions or in-situ reactions, which is vital and promising to develop novel sensor technologies.They will be used in fluorescent sensing systems for sensitive and selective detection of ions,biomolecules and cells in environmental and vital system by means of FRET (fluorescence resonance energy transfer), IFE (inner-filter effect) or other modes.These sensors will not only offer the optoelectronic advantages of conjugated polymers (such as strong fluorescence and signal amplification) and water solubility of electrolytes, but also provide properties of noble metal nanomaterials, which can efficiently eliminate the variable and ambiguous interference from light intensity, probe concentration, environmental conditions and instrumental efficiency, etc. Therefore, a technology platform based on these new nanocomposites and sensing modes will be established to sensitively and selectively obtain quantitative chemical and biological information in complex system, and which will expand the application of composite systems of conjugated polyelectrolytes and noble metal materials in disease diagnosis, genes mutations, environmental detection and national security.

英文关键词： Fluorescence;Chemo/bio sensors;Conjugated polyelectrolytes;Noble metal nanoparticles