PPR蛋白OsPPR920参与调控水稻花粉发育的分子机制研究

项目名称： PPR蛋白OsPPR920参与调控水稻花粉发育的分子机制研究

项目编号： No.31501375

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 刘玉君

作者单位： 浙江大学

项目金额： 20万元

中文摘要： 水稻是世界上最重要的粮食作物之一，而花发育及花粉育性的决定是其生命周期中的关键发育阶段。理解调控这一重要农艺性状的机理，既是植物生物学的重要基础研究课题，也是农业生产应用上的重要任务。本研究围绕一个参与调控水稻花粉发育的OsPPR920基因,分析其时空表达模式，获得OsPPR920 RNAi转基因株系及突变体材料进行表型分析，进行初步基因功能鉴定；深入解析OsPPR920蛋白在线粒体中的功能，明确其参与线粒体中哪一RNA分子转录后的何种生物学过程，从分子机理上揭示该蛋白对花粉发育的调控作用；通过验证OsPPR920与Osj10gBTF3蛋白的互作，并与调控途径中其它基因进行关联分析，初步探索 OsPPR920在Osj10gBTF3调控途径中的作用。本研究将阐明PPR蛋白OsPPR920参与调控水稻花粉发育的分子机制，进一步理解植物调控细胞质雄性不育及育性恢复的CMS/Rf系统的作用机理。

中文关键词： 作物；PPR蛋白；基因功能；花粉发育；分子机制

英文摘要： Rice is one of most important crops in the world, and male reproductive development and pollen fertility decision is a critical stage in its life cycle. Understanding the mechanism governing this process is both a fundamental question in plant biology and also an important task in agriculture application. In this study, we will focus on a rice male reproductive development related gene,OsPPR920, analyze its expression in different tissues and at different rice development stages, and create OsPPR920 RNAi transgenic lines and mutants for phenotype observation, to analyze the functions of OsPPR920 protein and its PPR motifs. Furthermore, OsPPR920 protein function in mitochondria will be investigated deeply, to reveal which mitochondrial RNA transcript and what biological process OsPPR920 protein involves in. Last, We will confirm the interaction between OsPPR920 and Osj10gBTF3 protein, and co-analyze the relationship between OsPPR920 and other genes in Osj10gBTF3 regulation pathway. These results will provide us preliminary cues for roles of OsPPR920 in Osj10gBTF3 regulation pathway. In short, this study will reveal the molecular mechanism of PPR protein OsPPR920 involved in rice male reproductive development process, to further understand the regulation mechanism of CMS/Rf system in plants.

英文关键词： Crop;PPR protein;Gene function;Pollen development;Molecular mechanism