暴马子皮苯乙醇苷类成分分离及其抗呼吸道合胞体病毒活性和作用机理研究

项目名称： 暴马子皮苯乙醇苷类成分分离及其抗呼吸道合胞体病毒活性和作用机理研究

项目编号： No.30873356

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 轻工业、手工业

项目作者： 马双成

作者单位： 中国食品药品检定研究院

项目金额： 33万元

中文摘要： 开发研制药效可靠、毒副作用小的防治呼吸道合胞体病毒（RSV）感染的药物，是目前国际上有待解决的重要课题之一。前期研究表明暴马子皮醇提物具有良好的抗RSV 作用，苯乙醇苷类成分为其抗RSV 有效部位。但其中的活性成分是什么？其作用机理如何？构效关系如何？目前尚不清楚。为此，本课题拟开展：（1）暴马子皮抗RSV 有效部位化学成分（苯乙醇苷类成分）分离与鉴定；（2）暴马子皮苯乙醇苷类成分抗RSV 活性评价与筛选；（3）暴马子皮抗RSV 活性成分作用机理及构效关系研究；（4）暴马子皮中抗RSV 活性成分对照品的制备与标定；（5）暴马子皮药材质量控制方法的研究。通过本课题的研究阐明暴马子皮抗RSV 药效物质，阐明暴马子皮抗RSV 活性成分及其作用机理和构效关系，为抗RSV 药物的发现提供依据，同时制备暴马子皮中活性成分的对照品，为暴马子皮药材的质量控制提供科学依据，填补暴马子皮药材质量控制研究空白。

中文关键词： 暴马子皮；苯乙醇苷类成分；抗呼吸道合胞体病毒；作用机理

英文摘要： It is one of very important international projects to research and develop the antiviral drugs against respiratory syncytial virus (RSV), the drugs have good pharmacodynamic actions and small sideeffects. Previous research results showed that the ethanol extract of the bark of Syringa reticulata had potent antiviral effects against RSV and the phenylethanoid glycosides was the effective parts against RSV, but it is not very clear what are the effective components, how about the mechanisms against RSV, and what is the structure-function relationships. This project will focus on the following research: (1) Isolation and structure elucidation of the components (phenylethanoid glycosides) from the effective parts of the bark of Syringa reticulata; (2) Antiviral evaluation and screening of the phenyletanoid glycosides isolated from Syringa reticulata against RSV; (3) Mechanisms research of the effective components against RSV and structure-function relationships; (4) Preparation and standardization of the reference standards, the effective components against RSV; (5) The quality control standard of the herbal drug Syringa reticulata. According to the above research, we hope to clarity the antiviral effective parts and components against RSV in Syringa reticulata, mechanisms, and structure-function relationship. Scientific evidence will be proved to develop antiviral drugs against RSV. Also, we can prepare the reference standards and formulate the quality control standard of Syringa reticulate. This research will supply the scientific evidence of the quality control of this herbal drug.

英文关键词： Syringa reticulata; phenylethanoid glycosides;antiviral effects against respiratory syncytial virus (RSV); mechanisms of action