超高压下大豆多糖-壳聚糖复合物形成机制及其功能表征

项目名称： 超高压下大豆多糖-壳聚糖复合物形成机制及其功能表征

项目编号： No.31501526

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 侯占群

作者单位： 中国食品发酵工业研究院有限公司

项目金额： 19万元

中文摘要： 超高压技术作为一种非热、有效、绿色的加工技术日益引起各国科研工作者重视。研究表明，超高压对蛋白质、多糖等生物大分子的空间结构及功能特性均有明显的改善，但对应用超高压处理天然多糖混合体系的相关研究尚未见报道。本项目以两种天然多糖-大豆多糖和壳聚糖为研究对象，借助FTIR、NMR、DSC、ITC、CLSM、 AFM、SEM、TEM等现代分析手段，系统研究多糖结构以及超高压处理条件对多糖复合物形成的影响，探讨复合物的流变学特性、微观结构、交联方式以及物理化学特性的变化，探明多糖分子交联方式及相互作用结合位点，揭示超高压下大豆多糖和壳聚糖复合物形成的作用机理。最终以复合物的乳化性、乳化稳定性等为主要指标，筛选大豆多糖和壳聚糖复合物的最佳形成条件，为进一步提高天然多糖使用价值，新型多糖复合物制备提供基础理论基础及数据支持。

中文关键词： 大豆多糖；壳聚糖；复合凝胶；功能特性；超高压

英文摘要： As a non- thermal, effective and green technology, ultra high pressure (UHP) treatment has been received great attention all over the world. Accumulated studies demonstrated that the physicochemical and functional properties of proteins and polysaccharides could be improved by the UHP treatment, however, there is still no information is available concerning the effect of UHP treatment on functional properties of mixed polysaccharides. Based on the previous studies, this proposal uses two natural polysaccharides, soybean soluble polysaccharides (SSPS) and chitosan (CHI) as raw materials. The FTIR、NMR、DSC、ITC、CLSM、AFM、SEM、TEM will be used to study the rheology properties, molecular structure,crosslinking mode and physicochemical properties of the SSPS/CHI compound under various conditions. This project is conducted to investigate the binding site between two the polysaccharides and illustrate the formation mechanism of SSPS/CHI compound under ultra high pressure. Finally, optimize the operation parameters to obtain multifunctional SSPS/CHI gels. This project is also expected to widen the range of potential application of natural polysaccharides, contribute significantly to the advancement in both the fundamental understanding and practical knowledge of the formation and production of novel food polysaccharides compounds.

英文关键词： soybean soluble polysaccharides;chitosan;compound gels;functional properties;ultra high pressure