红花黄色素生物合成相关R2R3-MYB转录因子基因的克隆、表达分析及其调控机制研究

项目名称： 红花黄色素生物合成相关R2R3-MYB转录因子基因的克隆、表达分析及其调控机制研究

项目编号： No.31201237

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 官丽莉

作者单位： 吉林农业大学

项目金额： 23万元

中文摘要： 红花黄色素（safflor yellow）是从传统中药红花中提取的主要有效成分之一。研究表明，红花黄色素具有扩张血管、抑制血栓形成、抗氧化等多种药理作用，被广泛地应用于冠心病、糖尿病并发症等疾病上，市场需求量较大。目前，虽然我国红花的种植面积在不断扩大，但仍未能解决红花黄色素资源匮乏问题。因此，提高红花自身黄色素含量，培育高产黄色素的红花新品系已迫在眉睫。本项目拟在红花花瓣转录物组测序基础上,分离红花黄色素生物合成相关R2R3-MYB转录因子CtMYB1基因，利用基因克隆技术获得该基因的全长cDNA片段，确证其表达特性、定位及转录激活特性；在此基础上，通过超表达和RNAi技术研究CtMYB1基因调控红花黄色素合成途径中结构基因的表达及黄色素的含量变化，进一步明确CtMYB1基因在红花黄色素合成中的调控机制，为通过基因工程手段培育高产黄色素红花新品系和开发红花黄色素的资源奠定基础。

中文关键词： 红花；黄色素；R2R3-MYB；代谢调控；转录因子

英文摘要： Safflor yellow is one of the main active ingredients extracted from safflower，which is a traditional Chinese medicine. Studies show that safflor yellow is in great demand on market, and applied widely in coronary heart disease, diabetes-related complications and other diseases, with many pharmacological effects, such as dilation of blood vessels,inhibition of thrombosis and antioxidation. At present, although safflower acreage continues to extend in China, there is still not able to resolve the resources demands of safflor yellow. Therefore,increasing the yield and breeding new variety with much higher production of safflor yellow is imminent. In this study, we will separate the regulatory gene (transcriptional factor R2R3-MYB) related to the regulation of safflor yellow biosynthesis;on the basis of safflower transcriptome sequencing, clone the full-length cDNA of CtMYB1 using gene cloning techniques, and research its expression characteristics, locations and transcription activities. Then we will further definite the role of CtMYB1 genes in regulation of safflor yellow biosynthesis by overexpression and knockdown of CtMYB1 genes, and lay a foundation for cultivating new safflower lines with high yield of safflor yellow and expanding the resources of safflor yellow by genetic engineering.

英文关键词： safflower；safflor yellow；R2R3-MYB；Metabolic regulation；Transctiption factor