基于核酸适体纳米传感新技术的靶分子信息识别与检测

项目名称： 基于核酸适体纳米传感新技术的靶分子信息识别与检测

项目编号： No.60801020

项目类型： 青年科学基金项目

立项/批准年度： 2009

项目学科： 金属学与金属工艺

项目作者： 王芳

作者单位： 武汉大学

项目金额： 22万元

中文摘要： 在本基金的资助下，我们开展了如下工作： 1）在常规电极表面构筑了几种具有信号放大功能的纳米界面，包括纳米铜-壳聚糖膜、纳米金-壳聚糖膜、碳纳米管-氧化镍膜、碳纳米管-纳米金膜等，实现了小分子的高效率催化，以此构建了相应的高灵敏度生物传感器，并成功应用于生物样品中靶标的分析，具有好的应用前景。 2）在碳纤维微电极表面构筑了纳米铜膜界面，并验证了其催化能力，实现了人血清中生物分子信息的快速检测，为传感器的微型化奠定了基础。 3）将核酸适体高度特异性分子识别的属性和电化学传感技术快速、高灵敏度的特点相结合，构筑了双功能免标记电化学核酸适体传感器用于识别和检测蛋白质凝血酶和小分子物质腺苷，并成功将其应用于人血浆中凝血酶和腺苷信息的分析。此类传感器将成为蛋白质等生物分子信息识别与检测的重要手段之一。 4）将几种纳米膜界面用于核酸适体传感器分析性能的提升，探索了新的固定基质及稳定的固定化方法，希望实现新型高通量电化学核酸适体生物传感器的构建。 5）中药活性成分分析的研究工作：荭草苷与异荭草苷的分离与检测及荭草苷与DNA分子的相互作用，探讨了电化学与色谱联用技术，为传感器微型化及联用应用奠定了基础。

中文关键词： 核酸适体；电化学检测；纳米生物传感；信号放大；药物分析。

英文摘要： We have done some research works as following in support of this funding： 1）Some nano-structured film modified conventional electrodes were fabricated including nano Cu-Chitosan, nano Au-Chitosan,CNTs-NiO, CNTs-nano Au. Their remarkable catalytic effect on oxidation of small molecules has been proved based on the signal amplification of nano-structured films,and then corresponding biosensors with high sensitivity were developed. 2）Nano-Cu particle modified carbon fiber micro-electrode was fabricated and was succesfully used to detect target molecule in human blood serum. 3）A bifunctional aptamer-based electrochemical biosensor for label-free detection of thrombin and adenosine has been developed based on the high specificity of aptamer and high sensitivity of electrochemical method.Finally, this sensor was successfully applied to thrombin and adenosine analysis in human plasma samples. 4）Fabricated nano-structured films were used to enhance the proformance of aptamer sensor and new immobilization method and material were investigated in order to construct novel electrochemical aptamer sensor with high-throughput. 5）Research on analysis of active components in traditional Chinese medicine: Simultaneous determination of orientin and isoorientin in Lophatherum gracile Brongn by high-performance liquid chromatography with electrochemical detectiondetection;Studies on electrochemical oxidation of orientin and its interaction with DNA.

英文关键词： Aptamer; Electrochemical detection; Nano-sensing technology;Signal amplification; Pharmaceutical analysis;