c-Abl基因缺失与PrPSc诱导神经元细胞氧化应激机制的研究

项目名称： c-Abl基因缺失与PrPSc诱导神经元细胞氧化应激机制的研究

项目编号： No.31272532

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 赵德明

作者单位： 中国农业大学

项目金额： 80万元

中文摘要： 朊病毒入侵宿主机体细胞后，可引起神经元细胞的氧化应激反应，最终引起细胞凋亡的机制仍不明确，诸多研究表明，氧化应激是Prion疾病发生的重要原因之一。非受体酪氨酸激酶在朊病毒感染细胞并诱导其凋亡通路中发挥重要作用。本研究利用shRNA干扰技术，制备c-abl基因缺失细胞株和基因缺失小鼠，从而抑制非受体酪氨酸激酶的表达和活化，以改善脑组织中神经元细胞在氧化应激条件下的抗凋亡水平。从体内、体外实验研究c-abl基因缺失对PrPSc诱导神经元细胞氧化应激的影响，进而为传染性海绵状脑病的治疗靶位点提供理论依据和技术支持。

中文关键词： 朊病毒疾病；神经元；氧化应激；非受体酪氨酸激酶；凋亡

英文摘要： The host cell of the body was infected with prion, it can cause the oxidative stress of the neurons, but the reason why it lead to apoptosis mechanism is still not clear. A number of studies have shown that the Oxidative stress of neurons is one of the most important reactions of the Prion disease. Non-receptor tyrosine kinase plays an important role in the apoptosis signaling pathway of Prion-infected cells. In this study, we will construct a c-abl gene deleted cell lines and gene-deficient mice through shRNA technology. Thus inhibites the expression and activation of non-receptor tyrosine kinase, so under the oxidative stress conditions in the brain, the ability of the neurons to resist apoptosis are changed. We do research about the c-abl gene deficiency can do what effect on the Oxidative stress of neurons directed by PrPSc in vitro and in vivo, that we can provide theoretical basis and technical support for the treatment of transmissible spongiform encephalopathies target sites.

英文关键词： Prion diseases；neuron；oxidative stress；C-Abl；apoptosis