深度聚集磁标记细胞的磁引导的研究

项目名称： 深度聚集磁标记细胞的磁引导的研究

项目编号： No.11304194

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 裴宁

作者单位： 上海大学

项目金额： 25万元

中文摘要： 在干细胞移植治疗心血管疾病中，人们发现不管采用何种移植途径，真正分布到心脏的移植细胞数量都极其有限。如何提高细胞的心脏归巢效率已成为干细胞疗法的难点。由于磁引导可以有效增加磁性微粒在组织的分布,为提高干细胞心脏归巢率，本课题组提出了磁引导磁标记干细胞归巢到心脏的方法。 由于常规的永磁铁或电磁铁产生的磁场具有远离磁极后磁场的强度迅速减少的特点，造成目前磁靶向治疗无法深度聚集磁性微粒。对较深的部位（心脏或肝等），传统的磁引导只能采用有创性手术将磁性材料植入组织。这极大增加了手术的风险。为克服传统磁导向的局限性，本课题组在自行研发的空间深度聚磁磁场装置的基础上设计出了空间深度聚集磁性微粒实验系统。本项目拟利用空间深度聚集磁性微粒实验系统全面研究磁场对磁标记骨髓间充质干细胞的深度聚集作用，获得磁引导磁标记细胞的规律。理论分析磁标记细胞在磁场作用下的动力学过程，完善磁引导磁标记细胞归巢心肌的机理。

中文关键词： 磁靶向；引导率；干细胞；；

英文摘要： Many studies concerning cell-based cardiac repair have shown that only a paucity of delivered cells are recruited at the infarct sites, irrespective of the delivery route used. Thus, how to improve the efficacy of cell transplantation for myocardial infarction has been recognized as a fundamental issue for cell-based therapies. We suggest magnetic strategy to localize magnetic labeled stem cells to heart to improve the capture efficiency for the retention of magnetic particles can be enhanced by using an external magnetic field. The magnetic field produced by the general magnet or electromagnet has some inherent limitations for the magnetic flux density is maximal at the magnet pole face and decreases dramatically at a distance from the magnet. So the general magnet or electromagnet cannot deep capture the magnetic particles at a distance from the magnet. Therefore, for deeper tissue such as myocardium or liver, invasive approach has been developed to implant magnetic materials at the target site to help attract and retain the magnetic particles. However, this method greatly increases the cost and risk of surgery. To overcome these limitations, we scheme out the equipment which spatially deep captures magnetic particles based on a self-made apparatus which spatial deep focused magnetic field. In this project, we

英文关键词： Magnetic targeting；capture efficiency；stem cell；；