基于拟南芥QTL位点RSB2的植物主茎分枝分子调控机制

项目名称： 基于拟南芥QTL位点RSB2的植物主茎分枝分子调控机制

项目编号： No.31471151

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 黄雪清

作者单位： 复旦大学

项目金额： 85万元

中文摘要： 高等植物的分枝发育决定着植物地上株型系统，在植物形态建成中占有十分重要的地位；植物的分枝也是影响作物生物量和产量的重要农艺性状。因此植物分枝的研究不仅具有重要的理论价值还可服务于农业生产，但目前对植物分枝调控的分子机制了解还较少。申请人在博士后期间运用国际上先进的多亲本重组自交系群体研究发现拟南芥中两个减少主茎分枝的主效QTL位点（RSB1和RSB2）加性互作共同调控主茎分枝， RSB1已被克隆鉴定为AGL6基因,但其调控分枝的分子机理仍不清楚；而RSB2定位于一个没有已知分枝调控基因的基因组区域，暗示它很可能是一个新基因。项目拟以RSB2 精细定位为出发点，用分子遗传学手段对RSB2进行克隆和鉴定。进一步综合运用生理生化，分子生物和组学等现代生物技术手段对RSB1和RSB2在植物分枝调控中的作用机理进行全面深入的研究，以期丰富植物调控分枝的特异性分子网络，为农业生产分子育种提供理论依据。

中文关键词： 拟南芥；分枝；数量性状位点；图位克隆；基因功能

英文摘要： Shoot branching is a major determinant of plant architecture, governing many aspects of form, light interception efficiency, and resource availability. Shoot branching enhances vegetative growth and generates multiple sites for seed production and may thereby affect the harvest index. Thus, the degree of branching can be a major determinant of plant biomass and seed yield. Studies on shoot branching not only has important theoretical value but can also serve in agricultural production. However, little is known about the specific molecular mechanisms underlying this trait. The applicant discovered 2 major quantitative trait loci (QTLs) for reduced stem branching (RSB1 and RSB2) epistatically regulate stem branching using international advanced Arabidopsis Multi-Parent Recombinant Inbred Line (AMPRIL) population during post-doctoral studying in Max-planck institute for plant research. RSB1 had been cloned and identified as the AGL6 gene but the molecular mechanism on regulating shoot branching is still not clear. RSB2 was mapped on a genomic region in which the known branching genes was not found, which suggests that RSB2 is most likely to be a new gene. In the project, based on the fine-mapping of RSB2, we will complete the cloning and identification of RSB2 using molecular genetic technology. In further, we will conduct a comprehensive study on the molecular mechanism of RSB1 and RSB2 on regulating shoot branching using several approaches such as molecular genetics, omics and biochemistry. The cloning and function research of RSB2 would improve our knowledge about shoot branching, and also could supply good candidate genes for molecular breeding of crops.

英文关键词： Arabidopsis thaliana;Shoot branching;Quantitative trait locus;Map-based cloning;Gene function