MYB转录因子对荔枝花色素苷降解的转录调控研究

项目名称： MYB转录因子对荔枝花色素苷降解的转录调控研究

项目编号： No.31301581

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 敬国兴

作者单位： 湘潭大学

项目金额： 23万元

中文摘要： 荔枝采后短时间内就发生褐变和腐烂变质，冷藏是最有效的保鲜方法，但低温贮藏的荔枝出库后常温货架期比未经冷藏的鲜果更短，褐变速率更快。国内外主要从酶促褐变的角度研究了荔枝中花色素苷的降解，但相关的分子机理尚未明确。本项目拟从MYB转录因子调控花色素苷降解中相关基因表达的角度研究荔枝中花色素苷迅速降解的现象，主要研究低温贮藏期和常温货架期中1）荔枝果皮花色素苷的含量变化；2）花色素苷酶（ANT）、多酚氧化酶（PPO）和过氧化物酶（POD）活性变化；3）MYB转录因子、ANT、PPO和POD基因的表达变化，和4）筛选具有转录调控ANT、PPO和POD基因表达的MYB转录因子。研究结果可望从分子生物学角度阐明花色素苷降解的机理，证实MYB转录因子在花色素苷降解过程中的转录调控作用。本项目研究成果在理论上可丰富花色素苷降解的发生发展机制；在实践上，为进一步探讨减缓荔枝果实花色素苷降解的技术提供新思路。

中文关键词： 荔枝；花色素苷；MYB转录因子；抗氧化；

英文摘要： Litchi (Litchi chinensis Sonn.) is a subtropical fruit with attractive red pericarp and translucent white aril. However, the fruit rapidly loses its red color during storage and becomes less attractive in the market, and the major limitation in litchi marketing is the rapid lose of the red color after harvest. Although litchi fruit can be stored for long time at low temperature, the fruit will deteriorate rapidly due to peel browning when expose to ambient temperature. Postharvest browning of litchi fruit was generally thought to be the results of rapid degradation of anthocyanins caused by the changeable activities of anthocyanase (ANT), peroxidase (POD) and polyphenol oxidase (PPO), but the molecular mechanism of it still remains unkown. It has been demonstrated that MYB proteins are key factors in regulatory networks controlling development, metabolism and responses to biotoic and abiotic stresses, and MYB transcription factor are important in controlling gene expressions of anthocyanin synthesis from the transcriptional level. Based on the previous studies, this study will (1) measure the concentration of anthocyanin, (2) investigate the changeable activities of anthocyanase (ANT), peroxidase (POD) and polyphenol oxidase (PPO) and (3)profile alterations of MYB transcription factor and gene expression of ANT,

英文关键词： Litchi；anthocyanins；MYB transcription factor；antioxidant；