人参皂苷Rg1在癫痫持续状态中的保护作用及分子机制

项目名称： 人参皂苷Rg1在癫痫持续状态中的保护作用及分子机制

项目编号： No.81460350

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 艾青龙

作者单位： 昆明医科大学

项目金额： 49万元

中文摘要： 癫痫持续状态（SE）中活性氧诱导神经损伤促进癫痫的进展和恶化，文献和前期研究中发现人参皂苷Rg1具有抗中枢神经系统炎症和氧化应激的药理作用，但人参皂苷Rg1在SE中的作用未见系统研究和报道。本课题拟通过构建氯化锂-匹罗卡品和海人酸诱导的癫痫持续状态大鼠模型和体外细胞模型，研究（1）人参皂苷Rg1对SE病理生理发生、发展过程中的调控作用；（2）人参皂苷Rg1对SE病理生理发生、发展过程中密切相关的关键信号通路磷脂酰肌醇3－激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白(PI3-K/ PKB/ mTOR)信号通路，有丝分裂原活化蛋白激酶(MAPKs)信号通路和核因子-κB (NF-κB)信号通路的调控作用，从分子、细胞及整体层面研究人参皂苷Rg1调控SE病理发生过程中的作用及其分子机制。为临床应用人参皂苷Rg1治疗SE提供分子生物学依据。

中文关键词： 人参皂苷Rg1；癫痫持续状态；活性氧；信号通路；匹罗卡品

英文摘要： Reactive oxygen species (ROS) induce nerve injury and promote the deterioration of epilepsy in status epilepticus (SE). Literature and preliminary studies indicated that ginsenoside Rg1 has potential of anti-inflammation and anti-oxidative stress in central nervous system. But the function of ginsenoside Rg1 in SE was few reported. This project intends to build SE rat model and related cell model by lithium-chloride/ pilocarpine and kainic acid respectively.The following aspects will be studied: (1) The pathophysiological role of ginsenosides Rg1 in the development of SE; (2) The regulation role of ginsenoside Rg1 in the key signaling pathways, including phosphatidylinositol 3 - kinase / protein kinase B / mammalian target of rapamycin signaling pathway (PI3-K / PKB / mTOR), mitogen-activated protein kinases signaling pathway (MAPKs) and nuclear factor-kappa B signaling pathway(NF-kappa B), which are closely related to the development process SE. So that the function and mechanisms of ginsenoside Rg1 in the pathologic process of SE would be further illuminated at the molecular,cellular and organic level respectively. And to provide moleculobiologic basis for ginsenoside Rg1 treantment of SE in clinic.

英文关键词： ginsenoside Rg1;status epilepticus;Reactive oxygen species;Signaling pathway;Pilocarpine