项目名称: 利用微纳电极对奥奈达希瓦氏菌胞外电子传递的研究
项目编号: No.31300113
项目类型: 青年科学基金项目
立项/批准年度: 2014
项目学科: 生物科学
项目作者: 彭罗
作者单位: 西南大学
项目金额: 22万元
中文摘要: 微生物胞外电子传递是众多生物地球化学过程的核心,也是多种新兴能源环境技术的基础。胞外电子传递中一些具体问题尚待研究。本项目将模式产电细菌奥奈达希瓦氏菌作为研究对象,探讨其间接电子传递中的介体扩散层厚度与分子扩散系数问题;以及胞外基质在直接电子传递中的作用。 试验引入微纳电极作为研究手段。试验涉及两种形式的微纳电极:微柱电极与纳米级刻蚀电极。微柱电极表面的扩散特性为实测电子介体的扩散层厚度与扩散系数提供了可能。而刻蚀电极便于单独研究胞外基质的电化学过程:包括胞外细胞色素的电化学行为(热力学,动力学),胞外细胞色素间的电子扩散,细菌能否通过胞外基质还原电极,特定细胞色素在上述过程中的作用。
中文关键词: 奥奈达希瓦氏菌;胞外电子传递;电子介体;细胞色素;纳米加工
英文摘要: Microbial extracellular electron transfer is central to numerous biogeochemical processes as well as a series of novel energy-environmental techniques. However, its underlying mechanisms are still obscure in many ways. With model exoelectrogen Shewanella oneidensis as the subject, this proposal aims to investigate how its extracellular matrix functions in the direct electron transfer. The proposal also targets at the issue of meditor diffusion layer thickness and diffusion coefficient. The study relies on micro/nanoelectrodes. Two types of micro/nanoelectrodes are involved: microcylinder electrodes and nanoelectrode fabricated by E-beam lithography. The characteristics of microcylinder enable the measurement of mediator diffusion coefficient and diffusion layer thickness. The nanoelectrode enables an exclusive investigation on the extracellular matrix, where the scope includes: electrochemical properties of extracelluar cytochromes (thermodynamics, kinetics); electron hopping among cytochromes; possible electron transfer via the matrix, as well as the role of particular cytochrome in the matrix.
英文关键词: Shewanella oneidensis;extracellular electron transfer;electron shuttle;cytochrome;nano-fabrication