转录因子SlWRKY6和SlWRKY41在硅诱导番茄抗旱性中的功能分析

项目名称： 转录因子SlWRKY6和SlWRKY41在硅诱导番茄抗旱性中的功能分析

项目编号： No.31501750

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 石玉

作者单位： 山西农业大学

项目金额： 20万元

中文摘要： 水分亏缺是旱作区番茄高产稳产的主要限制因素之一。通过使用外源硅（Si）可有效提高番茄的耐旱能力，但Si诱导番茄抗旱性的分子机制还不够清楚。WRKY转录因子与植物的抗逆应答反应密切相关，但番茄WRKY转录因子在水分亏缺下的调控功能尚未完全阐明。本项目以转录组测序结果为基础，以‘中杂9号’番茄为试材，基于RACE、real-time PCR、Southern blot、VIGS和转基因技术，克隆SlWRKY6和SlWRKY41基因并对其进行序列特性分析、基因家族分析、亚细胞定位与转录激活活性验证，构建SlWRKY6和SlWRKY41基因的不同表达活性植株，探讨这两个基因的时空表达特征及其与外源Si诱导番茄耐旱性的关系。本研究可望阐明外源Si诱导下SlWRKY6和SlWRKY41基因表达对水分亏缺的响应机制，为抗旱节水番茄育种提供理论依据，并探索一条基于Si肥的番茄抗旱高效栽培的新途径。

中文关键词： 番茄；水分亏缺；硅；转录因子；诱导抗性

英文摘要： Water deficit is one of the main factors limiting the stable and high tomato production in dry-farming areas. Application of exogenous silicon (Si) can effectively improve the drought resistance of tomato, but the molecular mechanism of Si-induced resistance has not been explored yet. WRKY transcription factors are closely related to the stress responses in plants. However, the regulatory function of tomato WRKY genes during water deficit has not been fully elucidated. Based on the transcriptome sequencing results, tomato cultivar ‘Zhongza No.9’ will be used as experimental material in this project. By means of RACE, real-time PCR and Southern blot, SlWRKY6 and SlWRKY41 genes will be cloned and then performed the sequence characteristics and gene family analysis, subcellular location and transactivation identification. Tomato plants with different SlWRKY6 and SlWRKY41 gene expressions will be constructed through VIGS and transgenic techniques. Moreover, the temporal and spatial changes of SlWRKY6 and SlWRKY41 genes expressions, and their relationship with exogenous Si-induced drought resistance in tomato will be investigated. The present study aims to clarify the response mechanisms of SlWRKY6 and SlWRKY41 transcription factors in the Si-mediated resistance of tomato to water deficit stress. All those will help to provide theoretical basis for tomato breeding concerned with drought resistance and water saving, and explore a new way based on Si fertilizer in the anti-drought and high-efficiency tomato cultivation .

英文关键词： tomato;water deficit;silicon;transcription factor;induced resistance