钛涂层表面生物功能化及其成骨性能的研究

项目名称： 钛涂层表面生物功能化及其成骨性能的研究

项目编号： No.31200718

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 生物物理、生化与生物分子学、生物力学与组织工程

项目作者： 吴晓东

作者单位： 中国人民解放军第二军医大学

项目金额： 23万元

中文摘要： 等离子体喷涂钛涂层具有优良的机械性能及粗糙多孔的表面结构，但钛不具有生物活性，植入体内后表面常覆盖了一层纤维膜，无骨组织长入。因此，对钛涂层进行表面改性以提高其生物活性是当前的研究热点。本项目前期研究中,用生物化学技术,在钛涂层表面共价接枝I型胶原。研究发现对碱处理后的钛涂层进行硅烷化处理，可引入活性氨基，利用交联剂激活I型胶原上的羧基，使之与氨基缩合，可形成肽键并将I型胶原有效固定于钛涂层表面。本项研究将在此基础上，共价接枝I型胶原和透明质酸，构建类细胞外基质，使之充当邻近骨细胞或基质的配体，促使骨细胞长入微孔，形成早期机械嵌合作用，有望从根本上改善钛涂层表面缺乏生物活性的问题。我们将考察骨细胞在涂层表面的黏附和生长情况及类细胞外基质对骨细胞及生物矿化的影响；用载体法负载细胞活性因子，考察细胞活性因子的释放过程及对骨细胞的影响；以体内实验研究涂层-骨组织界面的生物力学稳定性和骨整合情况。

中文关键词： 钛涂层；细胞外基质；I型胶原；透明质酸；生物化学改性

英文摘要： Plasma-sprayed titanium (Ti) coating has superior mechanical properties with multi-microporous structure on its surface. However，titanium is bioinert materials, the responses of bone to them are non-specific and the osseointegration into the microporous is poor. Therefore, current researches have focused on biochemical modification on the Ti coating to improve its bioactivity. Our initial experiments have already covalently immobilized fibrillar type-I collagen to the surfaces of the silanized Ti coating. If the Ti coating surfaces exhibit amino groups following silanization, the covalent attachment of collagen could be achieved via its carboxyl groups, which get activated by the cross-linking system. Following this biochemical method, our research is to covalently immobilize both fibrillar type-I collagen and hyaluronic acid to the surface of silanized Ti coating for reconstruction of extracellular matrix，and physically adsorpt recombinant human bone morphogenetic protein-2 (rhBMP-2). In vitro, cellular response of human bone marrow stromal osteoprogenitor cells (hBMSCs) to the modified Ti coating will be studied. In vivo, the biomechanical test and the histological experiment of implant-tissue integration will be studied. This research is armed to get a new bioactive Ti coating with better biocompatibility and

英文关键词： titanium coating；extracellular matrix；Type I collagen；hyaluronic acid；biochemical modification