三维孔结构石墨烯/纳米抗体复合电极制备及其在有机磷农药免疫传感上的应用

项目名称： 三维孔结构石墨烯/纳米抗体复合电极制备及其在有机磷农药免疫传感上的应用

项目编号： No.21505018

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 高分子科学

项目作者： 刘安然

作者单位： 东南大学

项目金额： 21万元

中文摘要： 本项目设计一种基于三维功能化石墨烯/纳米抗体的新型免疫传感功能界面，利用纳米抗体的高亲和力、强特异性及其与多孔功能化石墨烯的协同作用实现对有机磷农药小分子高灵敏度检测。本项目首先利用软/硬模板法在电极或固体基质表面原位构建具有优异导电性的三维功能化石墨烯孔结构；设计合成有机磷农药人工抗原，通过免疫双峰驼和后期基因转录获得对有机磷农药分子特异性识别的纳米抗体；实现纳米抗体在石墨烯孔结构上的高效组装与固定，研究电极、纳米功能界面、生物分子之间的电荷传输、物质迁移及电催化特性，以及抗体与界面的免疫识别能力；构建具有高灵敏度的光/电化学免疫传感器，并将不同有机磷农药纳米抗体分别固定在石墨烯多孔电极或固体基质表面，构建多种有机磷农药高通量传感阵列，实现对多组分有机磷农药分子的同时检测。本项目的完成将发展一种高效、简便的多组分有机磷农药残留的检测方法，具有较高的学术理论价值和应用前景。

中文关键词： 三维功能化石墨烯；纳米抗体；免疫传感器；有机磷农药

英文摘要： The principal objective of the program is to design a immunosensor based on 3D functionalized graphene / nanoantibody composites and to establish a fast, sensitive and high-throughput virus detection system. Firstly, functionalized graphene will be Controllable assembled onto conducting substrates by template methods and one-step electrochemical reduction to form three-dimensional interpenetrating graphene network with mesporous or macroporous structure. Secondly, five kinds of nanobodies will be constructed after immunizing an alpaca with artificial antigens prepared by haptens of organophosphorus pesticides. Thirdly, the obtained nanobodies with specific recognition of organophosphorus pesticide molecules will be assembled onto 3D functionalized graphene by covalent interaction or non-covalent interaction to construct a high sensitivity immunosensor to organophosphorus pesticides. Electron transfer and mass migration between biological sensitive interface and biomolecules will be studied by spectroscopic methods. Finally, several kinds of nanobodies will be immobilized on the 3D graphene arrays to fabricate the immune chip. The immune chip could simultaneously detect different components of organophosphorus pesticide mixture.

英文关键词： three-dimensional functionalized graphene;naonobody;immunosensor;organophosphorus pesticide