水稻组蛋白H2B泛素化修饰调控雄配子体发育的分子机制

项目名称： 水稻组蛋白H2B泛素化修饰调控雄配子体发育的分子机制

项目编号： No.31301052

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 曹红

作者单位： 中国科学院植物研究所

项目金额： 23万元

中文摘要： 在雄配子体发育过程中，基因的时空表达受严格的调控。组蛋白H2B单泛素化修饰的重要作用之一是基因转录调控，介导植物多个生物学过程，但在雄配子体发育中的功能尚不清楚。本项目主要用水稻缺失突变体来分析其修饰对水稻雄配子发育的调控机制。前期结果表明其修饰的关键成员OsHUB1和OsHUB2可能与雄配子体发育有关。其突变体hub1和hub2表现出结实率降低，花粉粒活力降低，部分三核期花粉粒的形态异常。本研究基于本课题组前期研究结果，拟利用遗传突变体、超表达和RNAi转基因水稻为材料，结合分子遗传学、细胞生物学、生物化学和转录组学等技术，探索水稻雄配子体发育过程中组蛋白泛素化修饰的调控机理。主要内容包括突变体和转基因材料的遗传与雄配子体细胞学分析、蛋白互作及其生化特征鉴定、E3连接酶作用模式与基因表达调控。本项目不仅对水稻雄配子体发育调控机制具有重要意义，而且将为水稻育种提供可能的理论基础。

中文关键词： 水稻；组蛋白修饰；泛素化；花药发育；转录调控

英文摘要： The development of male gametophyte is pivotal for fertilization and seed setting of crops. Some key genes are regulated temporally and spatially during male gametophyte development. Histone H2B monoubiquitination (H2Bub1) is of importance for chromatin remodeling and transcriptional regulation. H2Bub1 plays several roles in plants, but its function in male gametophyte development is unclear. The physiological function and molecular mechanism of H2Bub1 in rice is poorly understood. Here, loss of function mutants are used to studied the regulary mechanism of H2Bub1 on rice male gametophyte development. OsHUB1 and OsHUB2 (HISTONE UBIQUITINATION 1 and 2 in Oryza sativa) are putative E3 ligase of histone H2B ubiqutination of rice. Preliminary results indicated the two proteins of rice are related to male gametophyte development, as the rice hub1 and hub2 mutants displayed low seed setting rate, poor pollen activity, decreased number of pollen grains, and abnormal-shaped tricelcular stage microspores. In this study, mutants, overexpressed and RNAi transgenic rice of OsHUB1 and OsHUB2 are used as plant materials. The character of subcellular localization, protein interaction, expression pattern, biochemical properties of the two proteins are to be analyzed through cell biology, biochemistry, genetics and transcripto

英文关键词： rice；histone modification；ubiquitination；anther development；transcriptional regulation