基于磁性金属有机骨架材料的低丰度蛋白/肽段高效选择分离分析新方法研究

项目名称： 基于磁性金属有机骨架材料的低丰度蛋白/肽段高效选择分离分析新方法研究

项目编号： No.21505063

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 马荣娜

作者单位： 聊城大学

项目金额： 21万元

中文摘要： 低丰度生命分子研究已在临床标志物筛选和早期诊断领域发挥重要作用，而发展新技术与样品处理方法对于其快速高灵敏分析至关重要。金属有机骨架材料（MOFs）因其迷人的框架结构、高孔隙率等特性，广泛用于识别、催化、分离吸附等领域，而通过精确地可控设计可以在不改变其拓扑结构的前提下赋予其新的功能，为解决现存的生物分离、分析技术难题提供了新的切入点。本项目拟将磁性物理技术、材料技术、表面功能化及化学衍生与传统分析方法相结合，发展一系列生物样品高效特异性富集分析新方法。充分利用磁性MOFs的磁分离与高比表面积协同作用，通过可控的特定识别基团合成后修饰，制备适用于不同研究目的的新型功能磁性MOFs，为解决低丰度蛋白/肽段的特异性富集提供新颖的研究手段。并结合生物标记定量技术，集中于开发新型功能化磁性MOFs在低丰度目标分子高选择性分离富集及定量分析中的新应用，探索普适性的生命分子选择分离富集定量分析新策略。

中文关键词： 磁性金属有机骨架材料；生物功能化；选择性分离富集；定量分析；低丰度蛋白/肽段

英文摘要： Low abundance biomolecules has already taken a key place in clinical disease biomarkers screening, clinical diagnosis and therapy, etc. Therefore, it is necessary to develop new techniques and methodologies for the rapid and effective sample analysis. Metal organic frameworks (MOFs) have become one of the most exciting research topics and attracted considerable interest in recognition, catalysis, separation and adsorption because of its outstanding ultrahigh porosity, enormous internal surface areas and chemical properties. Especially, the precisely chemical modification can endow the specific functions without changing the underlying topology to afford a possibility for the bio-separation and technique. In this proposal，by combining magnetic physical technology, nanotechnology, surface functionalization, and chemical derivatization with traditional bioanalytical methods, a series of newly-designed bioanalytical methods to resolve existing problems in specific enrichment and detection biomolecules has been developed. This proposal made full use of the synergistic effect of magnetic MOFs from the two components of magnetic properties, highly ordered pores and special surface properties, through postsynthetic modification to synthesize several functional magnetic MOFs for different research requirements, and novel technologies have been introduced into low abundance proteome research fields. Furthermore, bio-tagging quantitative technology is combined for study the new application of magnetic MOFs in high selective separation, enrichment and quantitation of low abundance proteins and peptides. Finally, we aim at exploring and finding out widespread methodology in the selective enrichment and quantitation of target biomolecules. The application of MOFs in bio-analysis fields will not only open up a new possibility for the rapid analysis but also extend the application of MOFs mesoporous material.

英文关键词： Megnetic metal organic frameworks ;Biological functionalization;Selective separation and enrichment;Quantitative analysis;Low abundance proteins/peptides