MR成像检测动脉粥样硬化及心肌缺血梗死组织中Tenascin-C蛋白表达的实验研究

项目名称： MR成像检测动脉粥样硬化及心肌缺血梗死组织中Tenascin-C蛋白表达的实验研究

项目编号： No.81471723

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 马占龙

作者单位： 南京医科大学

项目金额： 70万元

中文摘要： 实现对动脉粥样硬化及急性心肌梗死发生、发展的共同的或相关的病理及分子生物学靶向成像检测，提高动脉粥样硬化及急性心肌缺血梗死发生、发展的早期诊断水平，是医学影像学重要的研究内容。本项目组开展MR靶向动脉粥样硬化成像研究多年，已经证实了MR靶向内皮祖细胞、巨噬细胞、LDL及合成型VSMCs的可行性。TN-C是动脉粥样硬化易损伤斑块及急性心肌缺血梗死组织中特异性高表达的糖蛋白，是研究动脉粥样硬化易损斑块与急性心肌缺血梗死发生、发展相关性的理想靶点。本项目拟以TN-C为研究靶点，制备特异性Fe2O3/DMSA-TN-C抗体探针。分别建立兔动脉粥样硬化模型及兔急性心肌缺血梗死模型，分别开展MR靶向成像检测动脉粥样硬化斑块及急性心肌缺血梗死组织中TN-C表达的研究。以期实现动脉粥样硬化斑块与急性心肌缺血梗死TN-C表达的在体MRI检测实验研究基础，促进对动脉粥样硬化及急性心肌梗死的早期靶向防治水平。

中文关键词： 磁共振成像；分子影像；动脉粥样硬化；心肌梗塞；Tenascin-C

英文摘要： Targeted molecular imaging to detect the pathological and biological process of atherosclerosis and acute infarction of myocardium in vivo, are considered to be important in clinical utilization. This research group have been years study of MR molecular imaging in atherosclerosis, and have successed in MR targeted imaging for endothelium progenitor cells(EPCs), macrophages, low-density lipoprotein(LDL) and the proliferated vascular smooth muscle cells(VSMCs). Tenascin-C(TN-C) is the attractive molecular imaging biomarkers for study the pathological and biological process of atherosclerosis and the infarction of myocardial, because it is a matricelluar protein and typicaly upregulated under the atherosclerotic plaque rupture and acute myocardial infarction. Therefore, based on the previous research work, this research project is desinged to develop the typical Fe2O3/DMSA-TN-C antibody MR imaging probe that bind to TN-C, and to performe the in vitro and in vivo MR imaging study for the express of TN-C respectively in atherosclerosis rabbits model and in the acute myocardial infarction rabbits model.We wish for make the basic research to visualize the expression of TN-C in the formation process of atherosclerostic plaque and myocardium infarction in vivo, and to improve the diagnosis and clinical targeted treatment for the cardiovascular disease.

英文关键词： MRI;Molecular imaging;Atherosclerosis;myocardial infarction;Tenascin-C