开窍药调控“神经血管单元”Wnt信号通路表达辛-心-开性效的机制研究

项目名称： 开窍药调控“神经血管单元”Wnt信号通路表达辛-心-开性效的机制研究

项目编号： No.81473371

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 王建

作者单位： 成都中医药大学

项目金额： 73万元

中文摘要： 前期研究显示，开窍药有抗脑缺血缺氧、抑制脑水肿、调节血脑屏障等作用而醒神回苏，具辛味-归心经-开窍醒神的性效构架，提出中药药性功能系统新学术观。缺血性脑卒中是一类致残率高的危重症。最新研究认为神经血管单元由神经元和血脑屏障构成，Wnt信号通路与之密切相关，在脑损伤修复中起重要作用，开窍药对Wnt通路研究甚少。本次提出开窍药通过调控神经血管单元及Wnt信号通路表达辛-心-开性效假说。采用激光共聚焦显微镜、免疫组化、免疫印迹、GC-MS等先进技术，融合多学科知识，围绕Wnt/β-catenin通路及Occludin、VEGF、EPO、HIF-1α、MMPS9等活性物质，从整体-器官-细胞多层次研究苏合香、冰片、安息香调控脑缺血模型大鼠神经血管单元Wnt信号通路的生物学机制，以揭示开窍药辛-心-开性效关联科学内涵，丰富中药药性理论。故有重要的学术价值和远期应用前景，亦具创新。

中文关键词： 中药药性；分子机制

英文摘要： According to the previous research, the orifice-opening medicine play a role in fighting againt the cerebral ischemia and hypoxia, inhibiting the brain edema, regulating the blood-brain barrier and etc. to induce resuscitation and restore consciousness. With an effect-frame pungent - staying in heart meridian - orifice-opening and resuscitation-inducing, the new academic idea Functional System in the Nature of Chinese Pharmacy has been proposed. The ischemic stroke is one of the critical illnesses leading to the high rate of disability. The latest research shows the neurovascular unit, consisting of neuron and blood-brain barrier, has a close connection with Wnt signal path and has an important effect in brain injury repairing. There are little study in the orifice-opening medicine functioning on Wnt path. This puts forward the hypothesis that the orifice-opening medicine has the efficiency in pungent-staying in heart meridian-orifice-opening and resuscitation-inducing by regulating and controlling the neurovascular unit and Wnt signal path. The advanced technologies such as CLSM,immunohistochemistry, immunoblotting and GC-MS will be applied and be combined with multi-disciplinary knowledge. Centering on Wnt/β-catenin path and active substances (such as Occludin、VEGF、EPO、HIF-1α、MMPS9), as well as researching the biological mechanisms from the multi-level entirety-organ-cell of the storax, borneol and benzoin in regulating and controlling the neurovascular unit and Wnt signal path of the rats with cerebral ischemia, it reveals the scientific connotation in the effect-relationship of pungent - staying in heart meridian - orifice-opening and resuscitation-inducing of the orifice-opening medicine and enriches the nature theory of Chinese pharmacy. So, it not only has significant academic value and future application prospect, but also with innovativeness.

英文关键词： Nature of Chinese pharmacy;Molecular mechanisms