E003调控滑膜细胞凋亡干预CIA大鼠发病的作用及机制研究

项目名称： E003调控滑膜细胞凋亡干预CIA大鼠发病的作用及机制研究

项目编号： No.31460239

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 傅颖媛

作者单位： 南昌大学

项目金额： 52万元

中文摘要： 滑膜细胞凋亡基因表达异常导致其凋亡受阻可能是类风湿关节炎患者关节滑膜慢性炎性增厚的重要原因。我们的前期研究发现， E003（即瑞香素）可明显抑制CIA大鼠（类风湿关节炎模型）免疫足肿胀及炎症，缓解关节软骨细胞退化变性,并可诱导其滑膜细胞凋亡。但目前对E003上述效应产生的细胞和分子机制知之甚少。本研究将应用E003作用CIA大鼠关节滑膜细胞和CIA大鼠模型，比较E003作用前后和凋亡信号传导途阻断剂作用前后，CIA大鼠滑膜细胞凋亡基因、信号传导通路基因mRNA和对应蛋白的表达情况，滑膜细胞凋亡率，以及动物体内疗效，从基因、分子、细胞、整体水平以及凋亡信号传导通路等多个层面，解析E003调控滑膜细胞凋亡干预CIA大鼠发病的作用及机制。本研究可为研制类风湿关节炎的新药及治疗提供理论和实验依据。

中文关键词： 调控机制；细胞凋亡；瑞香素；滑膜细胞；CIA

英文摘要： A resistance against programmed cell death induced by abnormal expression of apoptosis genes in synovial cell may be an important cause of synovial chronic inflammatory thickening in the patients with rheumatoid arthritis. Our previous studies have shown that E003 (daphnetin) significantly suppressed the immune foot swelling and inflammation of the collagen-induced arthritis (CIA, model of rheumatoid arthritis), alleviated the degeneration of articular cartilage cells and induced the apoptosis of synovial cells in CIA rats. However, the cellular and molecular mechanisms of the E003 acting on CIA rats are still poorly understood. In this study, the E003 will be respectively applied to CIA rat synovial cell and CIA rat model. In the presence and absence of E003, the expression of synovial cell apoptosis genes, apoptosis rate of synovial cell, the expression of mRNA and corresponding protein for signal transduction genes in CIA rats will be tested.In vivo the effect before and after the application of caspase inhibitor on apoptosis signal transduction pathway will be observed. This project from the aspects of aleration at various levels,such as mRNA,protern,cell and signal transduction pathways, will be used to analyze the regulation and its mechanism of E003 on apoptosis of synovial cells to intervene the pathogenesis of CIA rats. This study can provide theoretical and experimental basis for the development of new drugs and treatment of rheumatoid arthritis.

英文关键词： regulation mechanism;apotosis;Daphnetin;synovial cells;CIA