基于PCL和骨骼肌脱细胞基质构建三维打印静电纺丝组织工程支架修复腹壁缺损

项目名称： 基于PCL和骨骼肌脱细胞基质构建三维打印静电纺丝组织工程支架修复腹壁缺损

项目编号： No.81470792

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 顾岩

作者单位： 上海交通大学

项目金额： 73万元

中文摘要： 腹壁缺损的修复至今尚无一种理想材料，以组织工程材料进行腹壁缺损修复是腹壁外科发展方向。现有各种天然与合成可降解材料存在表面结构致密，细胞难以长入及存在模拟细胞外基质微环境的不足，而细胞的粘附、迁移、代谢以及早期血管化对内源性组织再生至关重要。因此，我们推测通过构建足够孔径与孔隙率及能够模拟细胞生存微环境的组织工程复合支架将能够显著改善腹壁缺损修复效果。我们预初研究发现三维打印技术能够精确调控具有良好生物学特性的聚己内酯(PCL)支架的孔径及孔隙率，而静电纺丝技术能够成功制备纳米级PCL/骨骼肌脱细胞基质(DMEM)支架改善细胞外微环境。因此，本课题拟首先构建PCL/DMEM复合支架，使三维打印PCL支架嵌于静电纺丝 PCL/DMEM层间，然后通过骨骼肌细胞种植构建组织工程复合支架，研究其促进新生血管和宿主细胞长入的效果与机制，结果将为构建精确可调控组织工程支架修复腹壁缺损提供重要理论指导。

中文关键词： 腹壁疝；腹壁缺损修复；组织工程；三维打印；静电纺丝

英文摘要： Abdominal wall defects are not, so far, repaired with an ideal material. The tissue engineering method is the main development direction for these defects repairment. Many natural and synthetic biodegradable materials, exhibits dense surface, which leads the host cells difficult to ingrowth the scaffold and have the deficiency of simulating extracellular matrix microenvironment. However, cell adhesion, migration, metabolism and early vascularization and host cells ingrowth play a critical role in endogenous tissue regeneration. So, we speculated that the composite scaffold with building the best pore size, porosity and simulating cell survival microenvironment would be able to significantly improve the effectiveness of abdominal wall defects. Our previous study found that three dimensional printing technology could be precisely controlled the pore size and porosity of the Polycaprolactone (PCL), which shows good biological and degradable property. And the electrostatic spinning technology could successfully build the nanoscale PCL/ Decellularized Muscular Extracellular Matrix (DMEM) composite scaffold to improve the cellular microenvironment.In order to study the mechanism of better early vascularization and host cells ingrowth about these scaffolds, we first build the PCL/DMEM composite scaffold by electrostatic spinning technology and the PCL scaffold by three dimensional printing technology, and make the PCL scaffold between the two PCL/DMEM composite scaffold, and then the muscle cells are implanted into its. The results will provide an important theoretical guidance for precisely regulating tissue engineering scaffold.

英文关键词： abdominal wall hernia;abdominal wall repairment;tissue engineering;three dimesional printing;electrostatic spinning