拟南芥AtSUC3和AtSUC9在蔗糖运输中的协同效应及机制研究

项目名称： 拟南芥AtSUC3和AtSUC9在蔗糖运输中的协同效应及机制研究

项目编号： No.30870190

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 建筑科学

项目作者： 张立军

作者单位： 沈阳农业大学

项目金额： 30万元

中文摘要： 大量研究表明，植物蔗糖转运蛋白在蛋白质水平存在着相互作用，但是目前尚缺乏有力的实验证明。我们的研究工作表明，拟南芥蔗糖转运蛋白AtSUC3和AtSUC9在蔗糖转运方面存在相互影响。拟南芥AtSUC3和AtSUC9基因的双敲除突变体的表现型，既不同于AtSUC3单基因敲除突变体，也不同于AtSUC9单基因敲除突变体。atsuc3/atsuc9双突变体与野生型、atsuc和atsuc9相比，种子萌发延迟、抽苔延迟、叶面积大，在营养期叶片含糖量降低，但在生殖期叶片糖含量高、花色素苷含量高。利用拟南芥离体愈伤组织的研究表明，atsuc3/atsuc9双突变含糖量、花色素苷含量也高于野生型和其它突变体。进一步的测定表明，atsuc3/atsuc9营养期叶片的糖含量高于生殖期，与野生型和2个单基因突变相反。RNA检测表明所研究的2个基因突变并不相互影响基因表达，说明它们之间的相互作用是在蛋白质水平或功能水平上。从上述结果中我们完全可以推测，AtSUC3主要起从叶肉细胞卸出蔗糖的作用。

中文关键词： 拟南芥；AtSUC3；AtSUC9；蔗糖；转运；相互作用

英文摘要： Recent studies show that the interaction exists between sucrose transporters at protein level, but lacking of powerful evidence to demonstrate this proposal. Our research work suggested that there exists a interaction between AtSUC3 and AtSUC9 from arobidopsis during transporting sucrose. The phenotype of atsuc3/atsuc9 double knockout plants is different from plants of atsuc3 knockout, atsuc9 knockout, and wild type. In comparison with atsuc3, atsuc9 and wild type, the atsuc3/atsuc9 plants show that seeds germinate later, plants bolt later, leaf area is larger, sucrse content is lower during vegetative stage and higher during reproduction stage in rosette leaf. At reproduction stage, atsuc3/atsuc9 rosette leaves also accummulate more anthocyanin. Data from the detached callus tissue culture show that atsuc3/atsuc9 accumulate more sucrose and anthocyanin. The further investigation shows that sucrose content in atsuc3/atsuc9 leaves is higher during reproduction stage than vegetative stage, which is in contrast to wild type plants, atsuc3, and atsuc9 mutants. RNA detests show that AtSUC3 gene or AtSUC9 gene knockout does not affect the other gene expression. This suggests that the reaction between AtSUC3 and AtSUC9 happens at protein level or function level. We can infer from the above results that AtSUC3 mainly mediates sucrose efflux from mesophyll cells.

英文关键词： arobidopsis; AtSUC3; AtSUC9; sucrose; transport; reaction