虫草蝠蛾耐寒性特征及机理研究

项目名称： 虫草蝠蛾耐寒性特征及机理研究

项目编号： No.31272366

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 张继红

作者单位： 中国科学院动物研究所

项目金额： 82万元

中文摘要： 蝠蛾是冬虫夏草的寄主昆虫，栖息于青藏高原的高寒地带，对低温形成了独特的适应性，是昆虫抗寒性研究的一个极好材料。但蝠蛾饲养困难、发育期长，已有的耐寒性研究非常有限，生理生化和分子生物学领域基本是空白。我们成功建立了虫草蝠蛾的室内人工种群，解决了实验材料来源这个关键问题。本项目将围绕耐寒性这一科学问题，阐明虫草蝠蛾的耐寒性特征，结合低温处理下差减杂交所获得的差异性表达基因和代谢小分子构建的代谢网络，揭示虫草蝠蛾抗寒系统应对低温环境时的整体调节路径，并在此基础上，寻找虫草蝠蛾抗寒性适应调节的关键代谢途径和具有调节功能的重要基因和酶，验证其在虫草蝠蛾抗寒性调节中所起的作用，阐明虫草蝠蛾抗寒的生理基础和分子机制, 揭示其耐寒性的本质。这项研究将加深人们对这种具有重要价值昆虫的基础生物学的认识，为人工栽培冬虫夏草提供理论和技术支持, 并可能在抗寒机制上有新的发现，丰富和发展昆虫抗寒性的理论和应用研究。

中文关键词： 蝙蝠蛾；冷适应；代谢组；能量代谢；热补偿

英文摘要： Hepialus spp., host of Ophiocordyceps sinensis (Berk.) G.H. Sung, J.M. Sung, Hywel-Jones & Spatafora, grown at the Tibetan Plateau from an altitude above 3,000 m up to the snow line and formed specific adaptation to the cold environment, is a very good material to study cold tolerance. However, with slow growth and great difficulty in arificial rearing, the research about the cold tolerance of Hepialus spp. is very limited. We have successfully set up the laboratory population of Hepialus armoricanus Oberthür, make it possible to provide sufficient larvae for the cold tolerance study. In this project, we are going to clarify the characterization of the cold tolerance of H. armoricanus, indicate the whole regulatory pathways of H. armoricanus toward the cold pressure by combining the results of differently expressed genes from SSH study and the metabolic networks of small molecular metabolites, upon these, find and test the roles of the key metabolic pathways and also the key genes and enzymes with important regulatory function in the anti-cold system of H. armoricanus. With the illuminations of the physiological bases and molecular mechanisms of cold responding system of H. armoricanus, this project will improves our knowledge toward the basic biology of this high economic value insect, provide theoretical and t

英文关键词： Ghost moths；Cold adaptation；Metabolomics；Energy metabolism；Thermal compensation